A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system

Winfried Edelmann, Burkhard Kröger, Martin Goller, Ivan Horak

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The recombinational frequency between two long terminal repeat elements (LTR-IS) of a mouse retrotransposon was about 13 times higher, compared with that of two control DNA sequences in extracts from mouse testes, but not in extracts from ascites cells. Deletion of a 37 bp region from the LTR-IS element strongly suppresses its recombinational activity. This 37 bp region encompasses an area of potentially single-stranded DNA and interacts with at least two nuclear proteins. One of them binds sequence-specifically to single-stranded DNA and is present in both types of extracts. Another protein(s) binds to dsDNA at the motif TGGAAATCCCC and is absent in extracts from testes. Our results suggest that a cis-acting DNA sequence within the 504 bp LTR-IS element is responsible for its high recombinational activity in vitro, and they further support the previous suggestion that the LTR-IS elements are meiotic recombinational hotspots in vivo.

Original languageEnglish (US)
Pages (from-to)937-946
Number of pages10
JournalCell
Volume57
Issue number6
DOIs
StatePublished - Jun 16 1989
Externally publishedYes

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Retroelements
DNA Transposable Elements
Genetic Recombination
Single-Stranded DNA
DNA sequences
Testis
Terminal Repeat Sequences
Nuclear Proteins
Cell Extracts
Ascites
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system. / Edelmann, Winfried; Kröger, Burkhard; Goller, Martin; Horak, Ivan.

In: Cell, Vol. 57, No. 6, 16.06.1989, p. 937-946.

Research output: Contribution to journalArticle

Edelmann, Winfried ; Kröger, Burkhard ; Goller, Martin ; Horak, Ivan. / A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system. In: Cell. 1989 ; Vol. 57, No. 6. pp. 937-946.
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