A recessive gene for primary vesicoureteral reflux maps to chromosome 12p11-q13

Patricia L. Weng, Simone Sanna-Cherchi, Terry Hensle, Ellen Shapiro, Alan Werzberger, Gianluca Caridi, Claudia Izzi, Anita Konka, Adam C. Reese, Rong Cheng, Samuel Werzberger, Richard N. Schlussel, Robert D. Burk, Joseph H. Lee, Roberto Ravazzolo, Francesco Scolari, Gian Marco Ghiggeri, Kenneth Glassberg, Ali G. Gharavi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Primary vesicoureteral reflux (pVUR) is one of the most common causes of pediatric kidney failure. Linkage scans suggest that pVUR is genetically heterogeneous with two loci on chromosomes 1p13 and 2q37 under autosomal dominant inheritance. Absence of pVUR in parents of affected individuals raises the possibility of a recessive contribution to pVUR. We performed a genome-wide linkage scan in 12 large families segregating pVUR, comprising 72 affected individuals. To avoid potential misspecification of the trait locus, we performed a parametric linkage analysis using both dominant and recessive models. Analysis under the dominant model yielded no signals across the entire genome. In contrast, we identified a unique linkage peak under the recessive model on chromosome 12p11-q13 (D12S1048), which we confirmed by fine mapping. This interval achieved a peak heterogeneity LOD score of 3.6 with 60% of families linked. This heterogeneity LOD score improved to 4.5 with exclusion of two high-density pedigrees that failed to link across the entire genome. The linkage signal on chromosome 12p11-q13 originated from pedigrees of varying ethnicity, suggesting that recessive inheritance of a high frequency risk allele occurs in pVUR kindreds from many different populations. In conclusion, this study identifies a major new locus for pVUR and suggests that in addition to genetic heterogeneity, recessive contributions should be considered in all pVUR genome scans.

Original languageEnglish (US)
Pages (from-to)1633-1640
Number of pages8
JournalJournal of the American Society of Nephrology
Volume20
Issue number7
DOIs
StatePublished - Jul 2009

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Recessive Genes
Vesico-Ureteral Reflux
Chromosomes
Genome
Pedigree
Genetic Heterogeneity
Gene Frequency
Renal Insufficiency
Pediatrics

ASJC Scopus subject areas

  • Nephrology

Cite this

Weng, P. L., Sanna-Cherchi, S., Hensle, T., Shapiro, E., Werzberger, A., Caridi, G., ... Gharavi, A. G. (2009). A recessive gene for primary vesicoureteral reflux maps to chromosome 12p11-q13. Journal of the American Society of Nephrology, 20(7), 1633-1640. https://doi.org/10.1681/ASN.2008111199

A recessive gene for primary vesicoureteral reflux maps to chromosome 12p11-q13. / Weng, Patricia L.; Sanna-Cherchi, Simone; Hensle, Terry; Shapiro, Ellen; Werzberger, Alan; Caridi, Gianluca; Izzi, Claudia; Konka, Anita; Reese, Adam C.; Cheng, Rong; Werzberger, Samuel; Schlussel, Richard N.; Burk, Robert D.; Lee, Joseph H.; Ravazzolo, Roberto; Scolari, Francesco; Ghiggeri, Gian Marco; Glassberg, Kenneth; Gharavi, Ali G.

In: Journal of the American Society of Nephrology, Vol. 20, No. 7, 07.2009, p. 1633-1640.

Research output: Contribution to journalArticle

Weng, PL, Sanna-Cherchi, S, Hensle, T, Shapiro, E, Werzberger, A, Caridi, G, Izzi, C, Konka, A, Reese, AC, Cheng, R, Werzberger, S, Schlussel, RN, Burk, RD, Lee, JH, Ravazzolo, R, Scolari, F, Ghiggeri, GM, Glassberg, K & Gharavi, AG 2009, 'A recessive gene for primary vesicoureteral reflux maps to chromosome 12p11-q13', Journal of the American Society of Nephrology, vol. 20, no. 7, pp. 1633-1640. https://doi.org/10.1681/ASN.2008111199
Weng, Patricia L. ; Sanna-Cherchi, Simone ; Hensle, Terry ; Shapiro, Ellen ; Werzberger, Alan ; Caridi, Gianluca ; Izzi, Claudia ; Konka, Anita ; Reese, Adam C. ; Cheng, Rong ; Werzberger, Samuel ; Schlussel, Richard N. ; Burk, Robert D. ; Lee, Joseph H. ; Ravazzolo, Roberto ; Scolari, Francesco ; Ghiggeri, Gian Marco ; Glassberg, Kenneth ; Gharavi, Ali G. / A recessive gene for primary vesicoureteral reflux maps to chromosome 12p11-q13. In: Journal of the American Society of Nephrology. 2009 ; Vol. 20, No. 7. pp. 1633-1640.
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