A putative SUMO interacting motif in the B30.2/SPRY domain of rhesus macaque TRIM5α important for NF-κB/AP-1 signaling and HIV-1 restriction

Marie Édith Nepveu-Traversy, Ann Demogines, Thomas Fricke, Mélodie B. Plourde, Kathleen Riopel, Maxime Veillette, Felipe Diaz-Griffero, Sara L. Sawyer, Lionel Berthoux

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

TRIM5α from the rhesus macaque (TRIM5αRh) is a restriction factor that shows strong activity against HIV-1. TRIM5αRhbinds specifically to HIV-1 capsid (CA) through its B30.2/PRYSPRY domain shortly after entry of the virus into the cytoplasm. Recently, three putative SUMO interacting motifs (SIMs) have been identified in the PRYSPRY domain of human and macaque TRIM5α. However, structural modeling of this domain suggested that two of them were buried in the hydrophobic core of the protein, implying that interaction with SUMO was implausible, while the third one was not relevant to restriction. In light of these results, we re-analyzed the TRIM5αRhPRYSPRY sequence and identified an additional putative SIM (435VIIC438) which we named SIM4. This motif is exposed at the surface of the PRYSPRY domain, allowing potential interactions with SUMO or SUMOylated proteins. Introducing a double mutation in SIM4 (V435K, I436K) did not alter stability, unlike mutations in SIM1. SIM4-mutated TRIM5αRhfailed to bind HIV-1CA and lost the ability to restrict this virus. Accordingly, SIM4 undergoes significant variation among primates and substituting this motif with naturally occurring SIM4 variants affected HIV-1 restriction by TRIM5αRh, suggesting a direct role in capsid recognition. Interestingly, SIM4-mutated TRIM5αRhalso failed to activate NF-κB and AP-1-mediated transcription. Although there is no direct evidence that SIM4 is involved in direct interaction with SUMO or a SUMOylated protein, mutating this motif strongly reduced co-localization of TRIM5αRhwith SUMO-1 and with PML, a SUMOylated nuclear protein. In conclusion, this new putative SIM is crucial for both direct interaction with incoming capsids and for NF-κB/AP-1 signaling. We speculate that the latter function is mediated by interactions of SIM4 with a SUMOylated protein involved in the NF-κB/AP-1 signaling pathways.

Original languageEnglish (US)
Article numbere00056
JournalHeliyon
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2016

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Transcription Factor AP-1
Macaca mulatta
HIV-1
Capsid
Virus Internalization
Amino Acid Motifs
Mutation
Proteins
Macaca
Nuclear Proteins
Primates
Cytoplasm
HIV
Viruses
B30.2-SPRY Domain

Keywords

  • Cellular aspects of innate immunity
  • Innate immune system
  • Proteins
  • Virology
  • Viruses

ASJC Scopus subject areas

  • General

Cite this

Nepveu-Traversy, M. É., Demogines, A., Fricke, T., Plourde, M. B., Riopel, K., Veillette, M., ... Berthoux, L. (2016). A putative SUMO interacting motif in the B30.2/SPRY domain of rhesus macaque TRIM5α important for NF-κB/AP-1 signaling and HIV-1 restriction. Heliyon, 2(1), [e00056]. https://doi.org/10.1016/j.heliyon.2015.e00056

A putative SUMO interacting motif in the B30.2/SPRY domain of rhesus macaque TRIM5α important for NF-κB/AP-1 signaling and HIV-1 restriction. / Nepveu-Traversy, Marie Édith; Demogines, Ann; Fricke, Thomas; Plourde, Mélodie B.; Riopel, Kathleen; Veillette, Maxime; Diaz-Griffero, Felipe; Sawyer, Sara L.; Berthoux, Lionel.

In: Heliyon, Vol. 2, No. 1, e00056, 01.01.2016.

Research output: Contribution to journalArticle

Nepveu-Traversy, MÉ, Demogines, A, Fricke, T, Plourde, MB, Riopel, K, Veillette, M, Diaz-Griffero, F, Sawyer, SL & Berthoux, L 2016, 'A putative SUMO interacting motif in the B30.2/SPRY domain of rhesus macaque TRIM5α important for NF-κB/AP-1 signaling and HIV-1 restriction', Heliyon, vol. 2, no. 1, e00056. https://doi.org/10.1016/j.heliyon.2015.e00056
Nepveu-Traversy, Marie Édith ; Demogines, Ann ; Fricke, Thomas ; Plourde, Mélodie B. ; Riopel, Kathleen ; Veillette, Maxime ; Diaz-Griffero, Felipe ; Sawyer, Sara L. ; Berthoux, Lionel. / A putative SUMO interacting motif in the B30.2/SPRY domain of rhesus macaque TRIM5α important for NF-κB/AP-1 signaling and HIV-1 restriction. In: Heliyon. 2016 ; Vol. 2, No. 1.
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abstract = "TRIM5α from the rhesus macaque (TRIM5αRh) is a restriction factor that shows strong activity against HIV-1. TRIM5αRhbinds specifically to HIV-1 capsid (CA) through its B30.2/PRYSPRY domain shortly after entry of the virus into the cytoplasm. Recently, three putative SUMO interacting motifs (SIMs) have been identified in the PRYSPRY domain of human and macaque TRIM5α. However, structural modeling of this domain suggested that two of them were buried in the hydrophobic core of the protein, implying that interaction with SUMO was implausible, while the third one was not relevant to restriction. In light of these results, we re-analyzed the TRIM5αRhPRYSPRY sequence and identified an additional putative SIM (435VIIC438) which we named SIM4. This motif is exposed at the surface of the PRYSPRY domain, allowing potential interactions with SUMO or SUMOylated proteins. Introducing a double mutation in SIM4 (V435K, I436K) did not alter stability, unlike mutations in SIM1. SIM4-mutated TRIM5αRhfailed to bind HIV-1CA and lost the ability to restrict this virus. Accordingly, SIM4 undergoes significant variation among primates and substituting this motif with naturally occurring SIM4 variants affected HIV-1 restriction by TRIM5αRh, suggesting a direct role in capsid recognition. Interestingly, SIM4-mutated TRIM5αRhalso failed to activate NF-κB and AP-1-mediated transcription. Although there is no direct evidence that SIM4 is involved in direct interaction with SUMO or a SUMOylated protein, mutating this motif strongly reduced co-localization of TRIM5αRhwith SUMO-1 and with PML, a SUMOylated nuclear protein. In conclusion, this new putative SIM is crucial for both direct interaction with incoming capsids and for NF-κB/AP-1 signaling. We speculate that the latter function is mediated by interactions of SIM4 with a SUMOylated protein involved in the NF-κB/AP-1 signaling pathways.",
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AU - Demogines, Ann

AU - Fricke, Thomas

AU - Plourde, Mélodie B.

AU - Riopel, Kathleen

AU - Veillette, Maxime

AU - Diaz-Griffero, Felipe

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AB - TRIM5α from the rhesus macaque (TRIM5αRh) is a restriction factor that shows strong activity against HIV-1. TRIM5αRhbinds specifically to HIV-1 capsid (CA) through its B30.2/PRYSPRY domain shortly after entry of the virus into the cytoplasm. Recently, three putative SUMO interacting motifs (SIMs) have been identified in the PRYSPRY domain of human and macaque TRIM5α. However, structural modeling of this domain suggested that two of them were buried in the hydrophobic core of the protein, implying that interaction with SUMO was implausible, while the third one was not relevant to restriction. In light of these results, we re-analyzed the TRIM5αRhPRYSPRY sequence and identified an additional putative SIM (435VIIC438) which we named SIM4. This motif is exposed at the surface of the PRYSPRY domain, allowing potential interactions with SUMO or SUMOylated proteins. Introducing a double mutation in SIM4 (V435K, I436K) did not alter stability, unlike mutations in SIM1. SIM4-mutated TRIM5αRhfailed to bind HIV-1CA and lost the ability to restrict this virus. Accordingly, SIM4 undergoes significant variation among primates and substituting this motif with naturally occurring SIM4 variants affected HIV-1 restriction by TRIM5αRh, suggesting a direct role in capsid recognition. Interestingly, SIM4-mutated TRIM5αRhalso failed to activate NF-κB and AP-1-mediated transcription. Although there is no direct evidence that SIM4 is involved in direct interaction with SUMO or a SUMOylated protein, mutating this motif strongly reduced co-localization of TRIM5αRhwith SUMO-1 and with PML, a SUMOylated nuclear protein. In conclusion, this new putative SIM is crucial for both direct interaction with incoming capsids and for NF-κB/AP-1 signaling. We speculate that the latter function is mediated by interactions of SIM4 with a SUMOylated protein involved in the NF-κB/AP-1 signaling pathways.

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