A pseudouridine residue in the spliceosome core is part of the filamentous growth program in yeast

Anindita Basak, Charles C. Query

Research output: Contribution to journalArticle

28 Scopus citations


Although pseudouridine nucleobases are abundant in tRNAs, rRNAs, and small nuclear RNAs (snRNAs), they are not known to have physiologic roles in cell differentiation. We have identified a pseudouridine residue (Ψ28) on spliceosomal U6 snRNA that is induced during filamentous growth of Saccharomyces cerevisiae. Pus1p catalyzes this modification and is upregulated during filamentation. Several U6 snRNA mutants are strongly pseudouridylated at Ψ28. Remarkably, these U6 mutants activate pseudohyphal growth, dependent upon Pus1p, arguing that U6-Ψ28 per se can initiate at least part of thefilamentous growth program. We confirmed this by using a designer small nucleolar RNA (snoRNA) targeting U6-U28 pseudouridylation. Conversely, mutants that block U6-U28 pseudouridylation inhibit pseudohyphal growth. U6-U28 pseudouridylation changes the splicing efficiency of suboptimal introns; thus, Pus1p-dependent pseudouridylation of U6 snRNA contributes to the filamentation growth program.

Original languageEnglish (US)
Pages (from-to)966-973
Number of pages8
JournalCell Reports
Issue number4
StatePublished - Aug 21 2014


ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this