A protocol for combining proliferation, tetramer staining and intracellular cytokine detection for the flow-cytometric analysis of antigen specific T-cells

Lidia Tesfa, H. D. Volk, F. Kern

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Flow-cytometry can be used in different ways in order to analyze or enumerate antigen specific T-cells. The three basic principles are direct staining of the T-cell receptor using so called tetramer reagents, staining intracellular cytokines following antigen-specific ex vivo T-cell activation or staining with dyes that are incorporated (increase in staining) or distributed between daughter cells (decrease in staining) upon proliferation in response to a specific antigen challenge. Each system has its advantages and disadvantages. Here we demonstrate that tetramer staining, cytokine flow cytometry and staining with CFDA-SE can be combined permitting the analysis of proliferation and cytokine production with a subset of T-cells specific for a single peptide antigen.

Original languageEnglish (US)
Pages (from-to)366-370
Number of pages5
JournalJournal of Biological Regulators and Homeostatic Agents
Volume17
Issue number4
StatePublished - Jan 1 2003
Externally publishedYes

Keywords

  • Antigen-specific T-cells
  • CFDA-SE
  • Cytokine flow cytometry
  • Tetramers

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Immunology and Allergy
  • Physiology
  • Immunology
  • Oncology
  • Endocrinology
  • Physiology (medical)
  • Cancer Research

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