Abstract
Flow-cytometry can be used in different ways in order to analyze or enumerate antigen specific T-cells. The three basic principles are direct staining of the T-cell receptor using so called tetramer reagents, staining intracellular cytokines following antigen-specific ex vivo T-cell activation or staining with dyes that are incorporated (increase in staining) or distributed between daughter cells (decrease in staining) upon proliferation in response to a specific antigen challenge. Each system has its advantages and disadvantages. Here we demonstrate that tetramer staining, cytokine flow cytometry and staining with CFDA-SE can be combined permitting the analysis of proliferation and cytokine production with a subset of T-cells specific for a single peptide antigen.
Original language | English (US) |
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Pages (from-to) | 366-370 |
Number of pages | 5 |
Journal | Journal of Biological Regulators and Homeostatic Agents |
Volume | 17 |
Issue number | 4 |
State | Published - 2003 |
Externally published | Yes |
Keywords
- Antigen-specific T-cells
- CFDA-SE
- Cytokine flow cytometry
- Tetramers
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Immunology and Allergy
- Physiology
- Immunology
- Oncology
- Endocrinology
- Physiology (medical)
- Cancer Research