TY - JOUR
T1 - A prospective study of telomere length measured by monochrome multiplex quantitative PCR and risk of lung cancer
AU - Shen, Min
AU - Cawthon, Richard
AU - Rothman, Nathaniel
AU - Weinstein, Stephanie J.
AU - Virtamo, Jarmo
AU - Hosgood, H. Dean
AU - Hu, Wei
AU - Lim, Unhee
AU - Albanes, Demetrius
AU - Lan, Qing
N1 - Funding Information:
This work was supported by intramural funds from the National Cancer Institute and extramural funds, including U.S. Public Health Service contracts N01-CN-45165, N01-RC-45035, and N01-RC-37004 from the National Cancer Institute, National Institutes of Health and Department of Health and Human Services .
PY - 2011/8
Y1 - 2011/8
N2 - Purpose: Telomere length plays an important role in chromosomal stability and tumorigenesis, and its measurement in peripheral white blood cell DNA may be a predictor of the development of lung cancer. Experimental design: Using a new method - monochrome multiplex quantitative PCR - which reduces measurement variability, we compared telomere length relative to standard DNA in white blood cell DNA in 229 incident male lung cancer cases and 229 matched controls within the prospective Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study of male smokers. Results: Median (10th, 90th percentile) telomere length was 1.13 (0.86, 1.45) in cases and 1.08 (0.85, 1.38) in controls (P=0.038). Telomere length was inversely associated with pack-years of smoking (Spearman's correlation r=-0.16, P=0.02) among controls. Compared to subjects with shorter telomere length (≤median), subjects with greater telomere length (>median) had a 1.6-fold (95% CI, 1.06-2.36) increased risk of lung cancer. There was a significant linear relationship between quartiles of telomere length and risk of lung cancer (odds ratios (95% confidence intervals) by quartile: 1.00, 0.98 (0.55-1.73), 1.62 (0.95-2.77), and 1.50 (0.84-2.68); P trend=0.05). In addition, subgroup analysis showed that greater telomere length was associated with an increased risk of lung cancer among longer-term smokers (>38 years) (OR, 1.90; 95% CI, 1.00-3.59) but not among shorter-term smokers (≤38 years) (OR, 1.08; 95% CI, 0.56-2.11) (P interaction=0.01). Conclusions: Our results suggest that greater telomere length may be associated with higher risk of lung cancer among male smokers.
AB - Purpose: Telomere length plays an important role in chromosomal stability and tumorigenesis, and its measurement in peripheral white blood cell DNA may be a predictor of the development of lung cancer. Experimental design: Using a new method - monochrome multiplex quantitative PCR - which reduces measurement variability, we compared telomere length relative to standard DNA in white blood cell DNA in 229 incident male lung cancer cases and 229 matched controls within the prospective Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study of male smokers. Results: Median (10th, 90th percentile) telomere length was 1.13 (0.86, 1.45) in cases and 1.08 (0.85, 1.38) in controls (P=0.038). Telomere length was inversely associated with pack-years of smoking (Spearman's correlation r=-0.16, P=0.02) among controls. Compared to subjects with shorter telomere length (≤median), subjects with greater telomere length (>median) had a 1.6-fold (95% CI, 1.06-2.36) increased risk of lung cancer. There was a significant linear relationship between quartiles of telomere length and risk of lung cancer (odds ratios (95% confidence intervals) by quartile: 1.00, 0.98 (0.55-1.73), 1.62 (0.95-2.77), and 1.50 (0.84-2.68); P trend=0.05). In addition, subgroup analysis showed that greater telomere length was associated with an increased risk of lung cancer among longer-term smokers (>38 years) (OR, 1.90; 95% CI, 1.00-3.59) but not among shorter-term smokers (≤38 years) (OR, 1.08; 95% CI, 0.56-2.11) (P interaction=0.01). Conclusions: Our results suggest that greater telomere length may be associated with higher risk of lung cancer among male smokers.
KW - Cohort study
KW - Lung cancer
KW - Telomere length
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U2 - 10.1016/j.lungcan.2010.11.009
DO - 10.1016/j.lungcan.2010.11.009
M3 - Article
C2 - 21507503
AN - SCOPUS:79960034290
SN - 0169-5002
VL - 73
SP - 133
EP - 137
JO - Lung Cancer
JF - Lung Cancer
IS - 2
ER -