A primary encephalocele culture yields a pure population of human astrocytes

Ron L. Alterman; Richard S. Morrison; James T. Goodrich; Peter Papenhausen; Joseph R. Moskal

doi:10.1016/0006-8993(91)91334-W

A primary encephalocele culture yields a pure population of human astrocytes

Ron L. Alterman, Richard S. Morrison, James T. Goodrich, Peter Papenhausen, Joseph R. Moskal

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

Original language	English (US)
Pages (from-to)	319-323
Number of pages	5
Journal	Brain research
Volume	550
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(91)91334-W
State	Published - Jun 7 1991

Keywords

Astrocyte
Encephalocele
Glia cell culture
Glial fibrillary acid protein
Growth factor

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(91)91334-W

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abstract = "In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.",

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AU - Alterman, Ron L.

AU - Morrison, Richard S.

AU - Goodrich, James T.

AU - Papenhausen, Peter

AU - Moskal, Joseph R.

PY - 1991/6/7

Y1 - 1991/6/7

N2 - In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

AB - In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

KW - Astrocyte

KW - Encephalocele

KW - Glia cell culture

KW - Glial fibrillary acid protein

KW - Growth factor

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A primary encephalocele culture yields a pure population of human astrocytes

Abstract

Keywords

ASJC Scopus subject areas

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