A primary encephalocele culture yields a pure population of human astrocytes

Ron L. Alterman, Richard S. Morrison, James Tait Goodrich, Peter Papenhausen, Joseph R. Moskal

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalBrain Research
Volume550
Issue number2
DOIs
StatePublished - Jun 7 1991

Fingerprint

Encephalocele
Astrocytes
Population
Glial Fibrillary Acidic Protein
Intercellular Signaling Peptides and Proteins

Keywords

  • Astrocyte
  • Encephalocele
  • Glia cell culture
  • Glial fibrillary acid protein
  • Growth factor

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

A primary encephalocele culture yields a pure population of human astrocytes. / Alterman, Ron L.; Morrison, Richard S.; Goodrich, James Tait; Papenhausen, Peter; Moskal, Joseph R.

In: Brain Research, Vol. 550, No. 2, 07.06.1991, p. 319-323.

Research output: Contribution to journalArticle

Alterman, Ron L. ; Morrison, Richard S. ; Goodrich, James Tait ; Papenhausen, Peter ; Moskal, Joseph R. / A primary encephalocele culture yields a pure population of human astrocytes. In: Brain Research. 1991 ; Vol. 550, No. 2. pp. 319-323.
@article{8ed929d77a1545c6a05baa539287608c,
title = "A primary encephalocele culture yields a pure population of human astrocytes",
abstract = "In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.",
keywords = "Astrocyte, Encephalocele, Glia cell culture, Glial fibrillary acid protein, Growth factor",
author = "Alterman, {Ron L.} and Morrison, {Richard S.} and Goodrich, {James Tait} and Peter Papenhausen and Moskal, {Joseph R.}",
year = "1991",
month = "6",
day = "7",
doi = "10.1016/0006-8993(91)91334-W",
language = "English (US)",
volume = "550",
pages = "319--323",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - A primary encephalocele culture yields a pure population of human astrocytes

AU - Alterman, Ron L.

AU - Morrison, Richard S.

AU - Goodrich, James Tait

AU - Papenhausen, Peter

AU - Moskal, Joseph R.

PY - 1991/6/7

Y1 - 1991/6/7

N2 - In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

AB - In order to develop a reliable source of human astrocytes for in vitro studies, we established a primary explant culture of a human encephalocele. This culture yielded a population of cells which were karyotypically normal, morphologically resembled astrocytes, expressed glial fibrillary acid protein, and responded mitogenically to exogenous growth factors. We conclude that white matter derived from human encephaloceles can be used to generate pure populations of normal astrocytes.

KW - Astrocyte

KW - Encephalocele

KW - Glia cell culture

KW - Glial fibrillary acid protein

KW - Growth factor

UR - http://www.scopus.com/inward/record.url?scp=0025824970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025824970&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(91)91334-W

DO - 10.1016/0006-8993(91)91334-W

M3 - Article

C2 - 1884239

AN - SCOPUS:0025824970

VL - 550

SP - 319

EP - 323

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -