A preparative method for the enzymatic 5′-monophosphorylation of nucleosides

David J. Merkler; Vern L. Schramm

doi:10.1016/0003-2697(87)90145-X

A preparative method for the enzymatic 5′-monophosphorylation of nucleosides

David J. Merkler, Vern L. Schramm

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [γ-³²P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.

Original language	English (US)
Pages (from-to)	148-153
Number of pages	6
Journal	Analytical Biochemistry
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(87)90145-X
State	Published - Nov 15 1987
Externally published	Yes

Keywords

adenosine kinase
nucleotide purification
nucleotide synthesis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(87)90145-X

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title = "A preparative method for the enzymatic 5′-monophosphorylation of nucleosides",

abstract = "A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [γ-32P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.",

keywords = "adenosine kinase, nucleotide purification, nucleotide synthesis",

author = "Merkler, {David J.} and Schramm, {Vern L.}",

note = "Funding Information: {\textquoteright} This work was supported by NIH Research Grants GM21083 and GM36604 (V.L.S.) and NIH Postdoctoral Fellowship GM10599 (D.J.M.). The NMR facility was supported by Equipment Grant RR02485 from the NIH. {\textquoteleft}Current address: Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 1046 1.",

year = "1987",

month = nov,

day = "15",

doi = "10.1016/0003-2697(87)90145-X",

language = "English (US)",

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pages = "148--153",

journal = "Analytical Biochemistry",

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T1 - A preparative method for the enzymatic 5′-monophosphorylation of nucleosides

AU - Merkler, David J.

AU - Schramm, Vern L.

N1 - Funding Information: ’ This work was supported by NIH Research Grants GM21083 and GM36604 (V.L.S.) and NIH Postdoctoral Fellowship GM10599 (D.J.M.). The NMR facility was supported by Equipment Grant RR02485 from the NIH. ‘Current address: Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 1046 1.

PY - 1987/11/15

Y1 - 1987/11/15

N2 - A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [γ-32P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.

AB - A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [γ-32P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.

KW - adenosine kinase

KW - nucleotide purification

KW - nucleotide synthesis

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DO - 10.1016/0003-2697(87)90145-X

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A preparative method for the enzymatic 5′-monophosphorylation of nucleosides

Abstract

Keywords

ASJC Scopus subject areas

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