A preparative method for the enzymatic 5′-monophosphorylation of nucleosides

David J. Merkler, Vern L. Schramm

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [γ-32P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalAnalytical Biochemistry
Volume167
Issue number1
DOIs
StatePublished - Nov 15 1987
Externally publishedYes

Keywords

  • adenosine kinase
  • nucleotide purification
  • nucleotide synthesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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