A predominantly nuclear protein affecting cytoplasmic localization of β-actin mRNA in fibroblasts and neurons

Wei Gu, Feng Pan, Honglai Zhang, Gary J. Bassell, Robert H. Singer

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

The localization of β-actin mRNA to the leading lamellae of chicken fibroblasts and neurite growth cones of developing neurons requires a 54-nt localization signal (the zipcode) within the 3′ untranslated region. In this study we have identified and isolated five proteins binding to the zipcode. One of these we previously identified as zipcode binding protein (ZBP)1, a 4-KH domain protein. A second is now investigated in detail: a 92-kD protein, ZBP2, that is especially abundant in extracts from embryonic brain. We show that ZBP2 is a homologue of the human hnRNP protein, KSRP, that appears to mediate premRNA splicing. However, ZBP2 has a 47-amino acid (aa) sequence not present in KSRP. Various portions of ZBP2 fused to GFP indicate that the protein most likely shuttles between the nucleus and the cytoplasm, and that the 47-aa insert promotes the nuclear localization. Expression of a truncated ZBP2 inhibits the localization of β-actin mRNA in both fibroblast and neurons. These data suggest that ZBP2, although predominantly a nuclear protein, has a role in the cytoplasmic localization of β-actin mRNA.

Original languageEnglish (US)
Pages (from-to)41-51
Number of pages11
JournalJournal of Cell Biology
Volume156
Issue number1
DOIs
StatePublished - Jan 7 2002

Fingerprint

Nuclear Proteins
Actins
Fibroblasts
Neurons
Messenger RNA
Heterogeneous-Nuclear Ribonucleoproteins
Growth Cones
3' Untranslated Regions
Neurites
Protein Binding
Amino Acid Sequence
Chickens
Carrier Proteins
Cytoplasm
Proteins
Amino Acids
Brain

Keywords

  • KH domain proteins
  • Nuclear-cytoplasmic trafficking
  • RNA binding proteins
  • RNA localization
  • RNA splicing

ASJC Scopus subject areas

  • Cell Biology

Cite this

A predominantly nuclear protein affecting cytoplasmic localization of β-actin mRNA in fibroblasts and neurons. / Gu, Wei; Pan, Feng; Zhang, Honglai; Bassell, Gary J.; Singer, Robert H.

In: Journal of Cell Biology, Vol. 156, No. 1, 07.01.2002, p. 41-51.

Research output: Contribution to journalArticle

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