A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization

Anne Christine Butty, Nathalie Perrinjaquet, Audrey Petit, Malika Jaquenoud, Jeffrey E. Segall, Kay Hofmann, Catherine Zwahlen, Matthias Peter

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, activation of Cdc42 by its guanine-nucleotide exchange factor Cdc24 triggers polarization of the actin cytoskeleton at bud emergence and in response to mating pheromones. The adaptor protein Beml localizes to sites of polarized growth where it interacts with Cdc42, Cdc24 and the PAK-like kinase Cla4. We have isolated Beml mutants (Bem1-m), which are specifically defective for binding to Cdc24. The mutations map within the conserved PB1 domain, which is necessary and sufficient to interact with the octicos peptide repeat (OPR) motif of Cdc24. Although Bem1-m mutant proteins localize normally, bem1-m cells are unable to maintain Cdc24 at sites of polarized growth. As a consequence, they are defective for apical bud growth and the formation of mating projections. Localization of Bem1 to the incipient bud site requires activated Cdc42, and conversely, expression of Cdc42-GTP is sufficient to accumulate Bem1 at the plasma membrane. Thus, our results suggest that Bem1 functions in a positive feed-back loop: local activation of Cdc24 produces Cdc42-GTP, which recruits Bem1. In turn, Bem1 stabilizes Cdc24 at the site of polarization, leading to apical growth.

Original languageEnglish (US)
Pages (from-to)1565-1576
Number of pages12
JournalEMBO Journal
Volume21
Issue number7
DOIs
StatePublished - Apr 2 2002
Externally publishedYes

Fingerprint

Guanine Nucleotide Exchange Factors
Polarization
Feedback
Growth
Guanosine Triphosphate
Chemical activation
p21-Activated Kinases
Pheromones
Mutant Proteins
Cell membranes
Actin Cytoskeleton
Yeast
Saccharomyces cerevisiae
Actins
Phosphotransferases
Cell Membrane
Peptides
Mutation
Proteins

Keywords

  • Actin
  • Bem1
  • Cdc24
  • Cell polarity
  • Polarized growth

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Butty, A. C., Perrinjaquet, N., Petit, A., Jaquenoud, M., Segall, J. E., Hofmann, K., ... Peter, M. (2002). A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. EMBO Journal, 21(7), 1565-1576. https://doi.org/10.1093/emboj/21.7.1565

A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. / Butty, Anne Christine; Perrinjaquet, Nathalie; Petit, Audrey; Jaquenoud, Malika; Segall, Jeffrey E.; Hofmann, Kay; Zwahlen, Catherine; Peter, Matthias.

In: EMBO Journal, Vol. 21, No. 7, 02.04.2002, p. 1565-1576.

Research output: Contribution to journalArticle

Butty, AC, Perrinjaquet, N, Petit, A, Jaquenoud, M, Segall, JE, Hofmann, K, Zwahlen, C & Peter, M 2002, 'A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization', EMBO Journal, vol. 21, no. 7, pp. 1565-1576. https://doi.org/10.1093/emboj/21.7.1565
Butty, Anne Christine ; Perrinjaquet, Nathalie ; Petit, Audrey ; Jaquenoud, Malika ; Segall, Jeffrey E. ; Hofmann, Kay ; Zwahlen, Catherine ; Peter, Matthias. / A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. In: EMBO Journal. 2002 ; Vol. 21, No. 7. pp. 1565-1576.
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