A population of rat liver lysosomes responsible for the selective uptake and degradation of cytosolic proteins

Ana Maria Cuervo, J. Fred Dice, Erwin Knecht

Research output: Contribution to journalArticle

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Abstract

Two populations of rat liver lysosomes can be distinguished on the basis of their density. A major difference between these populations is that one contains the heat shock cognate protein of 73 kDa (hsc73) within the lysosomal lumen. The lysosomal fraction containing hsc73 exhibits much higher efficiencies in the in vitro uptake and degradation of glyceraldehyde-3- phosphate dehydrogenase and ribonuclease A, two well established substrates of the selective lysosomal pathway of intracellular protein degradation. Preloading of the lysosomal population that is devoid of lumenal hsc73 with hsc73 isolated from cytosol activated the selective transport of substrate proteins into these lysosomes. Furthermore, treatment of animals with leupeptin, an inhibitor of lysosomal cathepsins, or 88 h of starvation also increased the amount of hsc73 within their lysosomal lumen, and these in vivo treatments also activated the selective transport of substrate proteins in vitro. Thus, the hsc73 located within lysosomes appears to be required for efficient uptake of cytosolic proteins by these organelles. The difference in hsc73 content between the lysosomal populations appears to be due to differences in their ability to take up hsc73 combined with differences in the intralysosomal degradation rates of hsc73. The increased stability of hsc73 in one population of lysosomes is primarily a consequence of this lysosomal population's more acidic pH.

Original languageEnglish (US)
Pages (from-to)5606-5615
Number of pages10
JournalJournal of Biological Chemistry
Volume272
Issue number9
DOIs
StatePublished - Feb 28 1997
Externally publishedYes

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Heat-Shock Proteins
Lysosomes
Liver
Proteolysis
Rats
Degradation
Population
Proteins
Carrier Proteins
Cathepsins
Pancreatic Ribonuclease
Glyceraldehyde-3-Phosphate Dehydrogenases
Substrates
Hot Temperature
Starvation
Organelles
Cytosol
Animals

ASJC Scopus subject areas

  • Biochemistry

Cite this

A population of rat liver lysosomes responsible for the selective uptake and degradation of cytosolic proteins. / Cuervo, Ana Maria; Dice, J. Fred; Knecht, Erwin.

In: Journal of Biological Chemistry, Vol. 272, No. 9, 28.02.1997, p. 5606-5615.

Research output: Contribution to journalArticle

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