TY - JOUR
T1 - A pneumococcal capsular polysaccharide vaccine induces a repertoire shift with increased V(H)3 expression in peripheral B cells from human immunodeficiency virus (HIV)-uninfected but not HIV-infected persons
AU - Chang, Q.
AU - Abadi, J.
AU - Alpert, P.
AU - Pirofski, Liise anne
PY - 2000
Y1 - 2000
N2 - The molecular mechanism of pneumococcal vaccine failure in human immunodeficiency virus (HIV)-infected persons is not fully understood. A polymerase chain reaction ELISA was used to determine the proportion of peripheral IgG, IgA, and IgM CD19-positive B cells expressing 6 immunoglobulin heavy-chain variable region (V(H)) subgroups before and 7 days after pneumococcal vaccination of 12 HIV-infected and 12 HIV-uninfected subjects. Significant postvaccination increases in the expression of the V(H)3 subgroup by IgG and IgA and a greater serologic response to vaccination were observed in the HIV-uninfected group. In contrast, the HIV-infected group had reduced prevaccination IgG V(H)3 and a postvaccination increase in IgG V(H)5. These results demonstrate that pneumococcal vaccination changes the pattern of B cell V(H) gene expression and support the concept that aberrant V(H)3 expression may translate into a poor antipneumococcal response in the setting of HIV infection.
AB - The molecular mechanism of pneumococcal vaccine failure in human immunodeficiency virus (HIV)-infected persons is not fully understood. A polymerase chain reaction ELISA was used to determine the proportion of peripheral IgG, IgA, and IgM CD19-positive B cells expressing 6 immunoglobulin heavy-chain variable region (V(H)) subgroups before and 7 days after pneumococcal vaccination of 12 HIV-infected and 12 HIV-uninfected subjects. Significant postvaccination increases in the expression of the V(H)3 subgroup by IgG and IgA and a greater serologic response to vaccination were observed in the HIV-uninfected group. In contrast, the HIV-infected group had reduced prevaccination IgG V(H)3 and a postvaccination increase in IgG V(H)5. These results demonstrate that pneumococcal vaccination changes the pattern of B cell V(H) gene expression and support the concept that aberrant V(H)3 expression may translate into a poor antipneumococcal response in the setting of HIV infection.
UR - http://www.scopus.com/inward/record.url?scp=0034022546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034022546&partnerID=8YFLogxK
U2 - 10.1086/315405
DO - 10.1086/315405
M3 - Article
C2 - 10762563
AN - SCOPUS:0034022546
SN - 0022-1899
VL - 181
SP - 1313
EP - 1321
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -