A photoaffinity probe that targets folate-binding proteins

Akihiro Takamura, Peter S. Thuy-Boun, Seiya Kitamura, Zhen Han, Dennis W. Wolan

Research output: Contribution to journalArticlepeer-review

Abstract

Folate and related derivatives are essential small molecules required for survival. Of significant interest is the biological role and necessity of folate in the crosstalk between commensal organisms and their respective hosts, including the tremendously complex human distal gut microbiome. Here, we designed a folate-based probe consisting of a photo-crosslinker to detect and quantitate folate-binding proteins from proteomic samples. We demonstrate the selectivity of our probe for the well-established human folate-binding protein dihydrofolate reductase and show no promiscuous labeling occurs with human caspase-3 or bovine serum albumin, which served as negative controls. Affinity-based enrichment of folate-binding proteins from an E. coli lysate in combination with mass spectrometry proteomics verified the ability of our probe to isolate low-abundance folate-dependent proteins. We envision that our probe will serve as a tool to elucidate the roles of commensal microbial folate-binding proteins in health and microbiome-related diseases.

Original languageEnglish (US)
Article number127903
JournalBioorganic and Medicinal Chemistry Letters
Volume40
DOIs
StatePublished - May 15 2021
Externally publishedYes

Keywords

  • Affinity-based photo-activatable probe
  • Folate
  • Folic acid
  • Mass spectrometry protein profiling
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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