A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye

Sun Yun Yu, Soon Ji Yoo, Lihui Yang, Cynthia Zapata, Anu Srinivasan, Bruce A. Hay, Nicholas E. Baker

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Regulated cell death and survival play important roles in neural development. Extracellular signals are presumed to regulate seven apparent caspases to determine the final structure of the nervous system. In the eye, the EGF receptor, Notch, and intact primary pigment and cone cells have been implicated in survival or death signals. An antibody raised against a peptide from human caspase 3 was used to investigate how extracellular signals controlled spatial patterning of cell death. The antibody crossreacted specifically with dying Drosophila cells and labelled the activated effector caspase Drice. It was found that the initiator caspase Dronc and the proapoptotic gene head involution defective were important for activation in vivo. Dronc may play roles in dying cells in addition to activating downstream effector caspases. Epistasis experiments ordered EGF receptor, Notch, and primary pigment and cone cells into a single pathway that affected caspase activity in pupal retina through hid and Inhibitor of Apoptosis Proteins. None of these extracellular signals appeared to act by initiating caspase activation independently of hid. Taken together, these findings indicate that in eye development spatial regulation of cell death and survival is integrated through a single intracellular pathway.

Original languageEnglish (US)
Pages (from-to)3269-3278
Number of pages10
JournalDevelopment
Volume129
Issue number13
StatePublished - 2002

Fingerprint

Initiator Caspases
Effector Caspases
Drosophila
Signal Transduction
Caspases
Cell Death
Epidermal Growth Factor Receptor
Cell Survival
Inhibitor of Apoptosis Proteins
Antibodies
Caspase 3
Nervous System
Retina
Head
Peptides
Survival
Genes

Keywords

  • Apoptosis
  • Casapse
  • Drice
  • Dronc
  • Drosophila eye
  • EGF receptor
  • Hid
  • IAP
  • Notch

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Yu, S. Y., Yoo, S. J., Yang, L., Zapata, C., Srinivasan, A., Hay, B. A., & Baker, N. E. (2002). A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye. Development, 129(13), 3269-3278.

A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye. / Yu, Sun Yun; Yoo, Soon Ji; Yang, Lihui; Zapata, Cynthia; Srinivasan, Anu; Hay, Bruce A.; Baker, Nicholas E.

In: Development, Vol. 129, No. 13, 2002, p. 3269-3278.

Research output: Contribution to journalArticle

Yu, SY, Yoo, SJ, Yang, L, Zapata, C, Srinivasan, A, Hay, BA & Baker, NE 2002, 'A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye', Development, vol. 129, no. 13, pp. 3269-3278.
Yu SY, Yoo SJ, Yang L, Zapata C, Srinivasan A, Hay BA et al. A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye. Development. 2002;129(13):3269-3278.
Yu, Sun Yun ; Yoo, Soon Ji ; Yang, Lihui ; Zapata, Cynthia ; Srinivasan, Anu ; Hay, Bruce A. ; Baker, Nicholas E. / A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye. In: Development. 2002 ; Vol. 129, No. 13. pp. 3269-3278.
@article{8d34e2afe7d4425082e3e8a3207b3b82,
title = "A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye",
abstract = "Regulated cell death and survival play important roles in neural development. Extracellular signals are presumed to regulate seven apparent caspases to determine the final structure of the nervous system. In the eye, the EGF receptor, Notch, and intact primary pigment and cone cells have been implicated in survival or death signals. An antibody raised against a peptide from human caspase 3 was used to investigate how extracellular signals controlled spatial patterning of cell death. The antibody crossreacted specifically with dying Drosophila cells and labelled the activated effector caspase Drice. It was found that the initiator caspase Dronc and the proapoptotic gene head involution defective were important for activation in vivo. Dronc may play roles in dying cells in addition to activating downstream effector caspases. Epistasis experiments ordered EGF receptor, Notch, and primary pigment and cone cells into a single pathway that affected caspase activity in pupal retina through hid and Inhibitor of Apoptosis Proteins. None of these extracellular signals appeared to act by initiating caspase activation independently of hid. Taken together, these findings indicate that in eye development spatial regulation of cell death and survival is integrated through a single intracellular pathway.",
keywords = "Apoptosis, Casapse, Drice, Dronc, Drosophila eye, EGF receptor, Hid, IAP, Notch",
author = "Yu, {Sun Yun} and Yoo, {Soon Ji} and Lihui Yang and Cynthia Zapata and Anu Srinivasan and Hay, {Bruce A.} and Baker, {Nicholas E.}",
year = "2002",
language = "English (US)",
volume = "129",
pages = "3269--3278",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "13",

}

TY - JOUR

T1 - A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye

AU - Yu, Sun Yun

AU - Yoo, Soon Ji

AU - Yang, Lihui

AU - Zapata, Cynthia

AU - Srinivasan, Anu

AU - Hay, Bruce A.

AU - Baker, Nicholas E.

PY - 2002

Y1 - 2002

N2 - Regulated cell death and survival play important roles in neural development. Extracellular signals are presumed to regulate seven apparent caspases to determine the final structure of the nervous system. In the eye, the EGF receptor, Notch, and intact primary pigment and cone cells have been implicated in survival or death signals. An antibody raised against a peptide from human caspase 3 was used to investigate how extracellular signals controlled spatial patterning of cell death. The antibody crossreacted specifically with dying Drosophila cells and labelled the activated effector caspase Drice. It was found that the initiator caspase Dronc and the proapoptotic gene head involution defective were important for activation in vivo. Dronc may play roles in dying cells in addition to activating downstream effector caspases. Epistasis experiments ordered EGF receptor, Notch, and primary pigment and cone cells into a single pathway that affected caspase activity in pupal retina through hid and Inhibitor of Apoptosis Proteins. None of these extracellular signals appeared to act by initiating caspase activation independently of hid. Taken together, these findings indicate that in eye development spatial regulation of cell death and survival is integrated through a single intracellular pathway.

AB - Regulated cell death and survival play important roles in neural development. Extracellular signals are presumed to regulate seven apparent caspases to determine the final structure of the nervous system. In the eye, the EGF receptor, Notch, and intact primary pigment and cone cells have been implicated in survival or death signals. An antibody raised against a peptide from human caspase 3 was used to investigate how extracellular signals controlled spatial patterning of cell death. The antibody crossreacted specifically with dying Drosophila cells and labelled the activated effector caspase Drice. It was found that the initiator caspase Dronc and the proapoptotic gene head involution defective were important for activation in vivo. Dronc may play roles in dying cells in addition to activating downstream effector caspases. Epistasis experiments ordered EGF receptor, Notch, and primary pigment and cone cells into a single pathway that affected caspase activity in pupal retina through hid and Inhibitor of Apoptosis Proteins. None of these extracellular signals appeared to act by initiating caspase activation independently of hid. Taken together, these findings indicate that in eye development spatial regulation of cell death and survival is integrated through a single intracellular pathway.

KW - Apoptosis

KW - Casapse

KW - Drice

KW - Dronc

KW - Drosophila eye

KW - EGF receptor

KW - Hid

KW - IAP

KW - Notch

UR - http://www.scopus.com/inward/record.url?scp=0036339935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036339935&partnerID=8YFLogxK

M3 - Article

VL - 129

SP - 3269

EP - 3278

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 13

ER -