A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors

Jeffrey Wyckoff, Weigang Wang, Elaine Y. Lin, Yarong Wang, Fiona Pixley, E. Richard Stanley, Thomas Graf, Jeffrey W. Pollard, Jeffrey E. Segall, John S. Condeelis

Research output: Contribution to journalArticle

692 Citations (Scopus)

Abstract

Invasion of tumor cells into the surrounding connective tissue and blood vessels is a key step in the metastatic spread of breast tumors. Although the presence of macrophages in primary tumors is associated with increased metastatic potential, the mechanistic basis for this observation is unknown. Using a chemotaxis-based in vivo invasion assay and multiphoton-based intravital imaging, we show that the interaction between macrophages and tumor cells facilitates the migration of carcinoma cells in the primary tumor. Gradients of either epidermal growth factor (EGF) or colony-stimulating factor 1 (CSF-1) stimulate collection into microneedles of tumor cells and macrophages even though tumor cells express only EGF receptor and macrophages express only CSF-1 receptor. Intravital imaging shows that macrophages and tumor cells migrate toward microneedles containing either EGF or CSF-1. Inhibition of either CSF-1- or EGF-stimulated signaling reduces the migration of both cell types. This work provides the first direct evidence for a synergistic interaction between macrophages and tumor cells during cell migration in vivo and indicates a mechanism for how macrophages may contribute to metastasis.

Original languageEnglish (US)
Pages (from-to)7022-7029
Number of pages8
JournalCancer Research
Volume64
Issue number19
DOIs
StatePublished - Oct 1 2004

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Cell Movement
Macrophages
Breast Neoplasms
Macrophage Colony-Stimulating Factor
Neoplasms
Epidermal Growth Factor
Colony-Stimulating Factor Receptors
Chemotaxis
Epidermal Growth Factor Receptor
Connective Tissue
Blood Vessels
Neoplasm Metastasis
Carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. / Wyckoff, Jeffrey; Wang, Weigang; Lin, Elaine Y.; Wang, Yarong; Pixley, Fiona; Stanley, E. Richard; Graf, Thomas; Pollard, Jeffrey W.; Segall, Jeffrey E.; Condeelis, John S.

In: Cancer Research, Vol. 64, No. 19, 01.10.2004, p. 7022-7029.

Research output: Contribution to journalArticle

Wyckoff, Jeffrey ; Wang, Weigang ; Lin, Elaine Y. ; Wang, Yarong ; Pixley, Fiona ; Stanley, E. Richard ; Graf, Thomas ; Pollard, Jeffrey W. ; Segall, Jeffrey E. ; Condeelis, John S. / A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. In: Cancer Research. 2004 ; Vol. 64, No. 19. pp. 7022-7029.
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