A Panel of Repeat Markers for Detection of Microsatellite Instability in Murine Tumors

Omar Kabbarah, Mary Ann Mallon, John D. Pfeifer, Winfried Edelmann, Raju Kucherlapati, Paul J. Goodfellow

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A substantial fraction of human malignancies lack functional DNA mismatch repair (MMR), accumulate mutations at high frequency, and exhibit microsatellite instability (MSI). In order to distinguish between MMR-competent and MMR-deficient malignancies, a consensus panel of microsatellite repeats has been adopted for MSI analysis of human tumors. There is no reference panel of repeats for MSI typing of murine malignancies. In this study, we present six new microsatellite repeat markers for MSI typing of mouse tumors. Analysis of polymerase chain reaction (PCR)-amplified tumor DNA from MMR-deficient and -proficient mice on denaturing polyacrylamide gels revealed that the new panel of microsatellites was more sensitive in detecting MSI than six commonly used CAn repeats. Using the new set of microsatellite markers, we demonstrated the presence of MSI in endometrial carcinoma and cancer precursors from diethylstilbestrol (DES)-treated mice, pointing to a possible role for loss of MMR in hormonally promoted endometrial tumorigenesis.

Original languageEnglish (US)
Pages (from-to)155-159
Number of pages5
JournalMolecular Carcinogenesis
Volume38
Issue number4
DOIs
StatePublished - Dec 2003

Keywords

  • Diethylstilbestrol
  • Endometrial cancer
  • MMR
  • MSI

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

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    Kabbarah, O., Mallon, M. A., Pfeifer, J. D., Edelmann, W., Kucherlapati, R., & Goodfellow, P. J. (2003). A Panel of Repeat Markers for Detection of Microsatellite Instability in Murine Tumors. Molecular Carcinogenesis, 38(4), 155-159. https://doi.org/10.1002/mc.10157