A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae

Erin A. Powrie, Daniel Zenklusen, Robert H. Singer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The biogenesis of a localization-competent mRNP begins in the nucleus. It is thought that the coordinated action of nuclear and cytoplasmic components of the localization machinery is required for the efficient export and subsequent subcellular localization of these mRNAs in the cytoplasm. Using quantitative poly(A)+ and transcript-specific fluorescent in situ hybridization, we analyzed different nonessential nucleoporins and nuclear pore-associated proteins for their potential role in mRNA export and localization. We found that Nup60p, a nuclear pore protein located on the nucleoplasmic side of the nuclear pore complex, was required for the mRNA localization pathway. In a δnup60 background, localized mRNAs were preferentially retained within the nucleus compared to nonlocalized transcripts. However, the export block was only partial and some transcripts could still reach the cytoplasm. Importantly, downstream processes were also affected. Localization of ASH1 and IST2 mRNAs to the bud was impaired in the δnup60 background, suggesting that the assembly of a localization competent mRNP ("locasome") was inhibited when NUP60 was deleted. These results demonstrate transcript specificity of a nuclear mRNA retention defect and identify a specific nucleoporin as a functional component of the localization pathway in budding yeast.

Original languageEnglish (US)
Pages (from-to)134-144
Number of pages11
JournalRNA
Volume17
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Nuclear Pore Complex Proteins
Saccharomyces cerevisiae
Nuclear Pore
Messenger RNA
Porins
Nuclear Proteins
Cytoplasm
Poly A
Saccharomycetales
Fluorescence In Situ Hybridization

Keywords

  • ASH1
  • In situ hybridization
  • mRNA export
  • NUP60
  • RNA localization

ASJC Scopus subject areas

  • Molecular Biology

Cite this

A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae. / Powrie, Erin A.; Zenklusen, Daniel; Singer, Robert H.

In: RNA, Vol. 17, No. 1, 01.2011, p. 134-144.

Research output: Contribution to journalArticle

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