A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells

Tatsuya Nakamura, Jana Hillova, Régine Mariage-Samson, Myriam Onno, Kay Huebner, Linda A. Cannizzaro, Leslie Boghosian-Sell, Carlo M. Croce, Miroslav Hill

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Tre is a recombinant gene isolated from NIH3T3 cells transfected with human Ewing's sarcoma DNA. It is composed of three major genetic elements derived, 5′ to 3′, from human chromosomes 5, 18 and 17. We report here on transcripts from the 3′ domain of tre. The transcripts were cloned from a cDNA library of cytoplasmic poly(A)+ RNA from tri-transfected NIH3T3 tumor cells. The complete cDNA sequence, 8201 nucleotides, possessed an unusually long non-coding region and a translatable region with two open reading frames. In one cDNA clone, the presence of two insertions suggested the possibility of alternative splicing. The sequence mapped to the centromere-proximal region of 17q. Transfection-tumorigenicity assays with the open reading frames subcloned into expression vectors were positive for the reading frame adjacent to the 5′ noncoding region and negative for the second, downstream, reading frame and the possible alternatively spliced versions of both reading frames. Analysis of the 786 amino acid sequence deduced from the 5′ reading frame predicted a highly hydrophilic protein with two charge clusters suggesting nucleic acid-binding properties. When used as probe, the cloned sequence detected RNA transcripts in a wide variety of human cancer cells regardless of their lineage of origin from different tissues, but not in human cells from normal tissue.

Original languageEnglish (US)
Pages (from-to)733-741
Number of pages9
JournalOncogene
Volume7
Issue number4
StatePublished - Apr 1992
Externally publishedYes

Fingerprint

Reading Frames
Oncogenes
Open Reading Frames
Neoplasms
Complementary DNA
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 17
Ewing's Sarcoma
Centromere
Protein Sequence Analysis
Alternative Splicing
Human Chromosomes
Gene Library
Nucleic Acids
Transfection
Clone Cells
Messenger RNA
DNA
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Nakamura, T., Hillova, J., Mariage-Samson, R., Onno, M., Huebner, K., Cannizzaro, L. A., ... Hill, M. (1992). A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. Oncogene, 7(4), 733-741.

A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. / Nakamura, Tatsuya; Hillova, Jana; Mariage-Samson, Régine; Onno, Myriam; Huebner, Kay; Cannizzaro, Linda A.; Boghosian-Sell, Leslie; Croce, Carlo M.; Hill, Miroslav.

In: Oncogene, Vol. 7, No. 4, 04.1992, p. 733-741.

Research output: Contribution to journalArticle

Nakamura, T, Hillova, J, Mariage-Samson, R, Onno, M, Huebner, K, Cannizzaro, LA, Boghosian-Sell, L, Croce, CM & Hill, M 1992, 'A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells', Oncogene, vol. 7, no. 4, pp. 733-741.
Nakamura T, Hillova J, Mariage-Samson R, Onno M, Huebner K, Cannizzaro LA et al. A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. Oncogene. 1992 Apr;7(4):733-741.
Nakamura, Tatsuya ; Hillova, Jana ; Mariage-Samson, Régine ; Onno, Myriam ; Huebner, Kay ; Cannizzaro, Linda A. ; Boghosian-Sell, Leslie ; Croce, Carlo M. ; Hill, Miroslav. / A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. In: Oncogene. 1992 ; Vol. 7, No. 4. pp. 733-741.
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