A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

Walter J. Miller; Mark L. Kayton; Angela Patton; Sarah O'Connor; Mei He; Huan Vu; Galina Baibakov; Dominique Lorang; Vladimir Knezevic; Elise Kohn; H. Richard Alexander; David Stirling; Faribourz Payvandi; George W. Muller; Steven K. Libutti

doi:10.1186/1479-5876-2-4

A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

Walter J. Miller, Mark L. Kayton, Angela Patton, Sarah O'Connor, Mei He, Huan Vu, Galina Baibakov, Dominique Lorang, Vladimir Knezevic, Elise Kohn, H. Richard Alexander, David Stirling, Faribourz Payvandi, George W. Muller, Steven K. Libutti

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response.

Original language	English (US)
Article number	4
Journal	Journal of Translational Medicine
Volume	2
DOIs	https://doi.org/10.1186/1479-5876-2-4
State	Published - Jan 30 2004
Externally published	Yes

Keywords

Angiogenesis
Chorioallantoic membrane (CAM)
Confocal microscopy
Layered Expression Scanning (LES)
XTT assay

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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Miller, W. J., Kayton, M. L., Patton, A., O'Connor, S., He, M., Vu, H., Baibakov, G., Lorang, D., Knezevic, V., Kohn, E., Alexander, H. R., Stirling, D., Payvandi, F., Muller, G. W., & Libutti, S. K. (2004). A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay. Journal of Translational Medicine, 2, [4]. https://doi.org/10.1186/1479-5876-2-4

A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay. / Miller, Walter J.; Kayton, Mark L.; Patton, Angela et al.

In: Journal of Translational Medicine, Vol. 2, 4, 30.01.2004.

Research output: Contribution to journal › Article › peer-review

Miller, WJ, Kayton, ML, Patton, A, O'Connor, S, He, M, Vu, H, Baibakov, G, Lorang, D, Knezevic, V, Kohn, E, Alexander, HR, Stirling, D, Payvandi, F, Muller, GW & Libutti, SK 2004, 'A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay', Journal of Translational Medicine, vol. 2, 4. https://doi.org/10.1186/1479-5876-2-4

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abstract = "Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response.",

keywords = "Angiogenesis, Chorioallantoic membrane (CAM), Confocal microscopy, Layered Expression Scanning (LES), XTT assay",

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AU - Miller, Walter J.

AU - Kayton, Mark L.

AU - Patton, Angela

AU - O'Connor, Sarah

AU - He, Mei

AU - Vu, Huan

AU - Baibakov, Galina

AU - Lorang, Dominique

AU - Knezevic, Vladimir

AU - Kohn, Elise

AU - Alexander, H. Richard

AU - Stirling, David

AU - Payvandi, Faribourz

AU - Muller, George W.

AU - Libutti, Steven K.

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AB - Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response.

KW - Angiogenesis

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KW - Confocal microscopy

KW - Layered Expression Scanning (LES)

KW - XTT assay

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A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

Abstract

Keywords

ASJC Scopus subject areas

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