A novel papillomavirus isolated from proliferative skin lesions of a wild American beaver (Castor canadensis)

Artem S. Rogovskyy, Timothy V. Baszler, Daniel S. Bradway, Darren L. Bruning, Christine M. Davitt, James F. Evermann, Robert D. Burk, Zigui Chen, Kristin G. Mansfield, Gary J. Haldorson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cutaneous papillomatosis was diagnosed in an adult American beaver (Castor canadensis). Gross lesions included numerous exophytic, roughly circular, lightly pigmented lesions on hairless areas of fore and hind feet and the nose. The most significant histopathologic findings were multifocal papilliform hyperplasia of the superficial stratified squamous epithelium, with multifocal koilocytes, and multiple cells with large, darkly basophilic intranuclear inclusion bodies. A virus with properties consistent with papillomavirus (PV) was recovered by virus isolation of skin lesions, utilizing rabbit and feline kidney cell lines. The presence of the virus was confirmed by PV-specific polymerase chain reaction. The partial sequences of E1 and L1 genes did not closely match those of any PVs in GenBank, suggesting that this might be a new type of PV. Partial E1 and L1 nucleotide sequences of the beaver papillomavirus (hereafter, ARbeaver-PV1) were used to create a phylogenetic tree employing the complete E1 and L1 open reading frame nucleotide sequences of 68 PVs. The phylogenetic tree placed the ARbeaver-PV1 in a clade that included the Mupapillomavirus (HPV1 and HPV63) and Kappapapillomavirus (OcPV1 and SfPV1) genera. The present article confirms the papillomaviral etiology of cutaneous exophytic lesions in the beaver.

Original languageEnglish (US)
Pages (from-to)750-754
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number4
DOIs
StatePublished - Jul 2012

Fingerprint

Castor canadensis
Papillomaviridae
skin lesions
Rodentia
Kappapapillomavirus
Mupapillomavirus
Viruses
Skin
Castoridae
lesions (animal)
viruses
Intranuclear Inclusion Bodies
Felidae
Nucleic Acid Databases
Papilloma
nuclear inclusions
Nose
nucleotide sequences
Open Reading Frames
papilloma

Keywords

  • American beavers
  • Castor canadensis
  • cutaneous papillomatosis
  • papillomatosis
  • papillomavirus
  • proliferative skin lesion

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Rogovskyy, A. S., Baszler, T. V., Bradway, D. S., Bruning, D. L., Davitt, C. M., Evermann, J. F., ... Haldorson, G. J. (2012). A novel papillomavirus isolated from proliferative skin lesions of a wild American beaver (Castor canadensis). Journal of Veterinary Diagnostic Investigation, 24(4), 750-754. https://doi.org/10.1177/1040638712448654

A novel papillomavirus isolated from proliferative skin lesions of a wild American beaver (Castor canadensis). / Rogovskyy, Artem S.; Baszler, Timothy V.; Bradway, Daniel S.; Bruning, Darren L.; Davitt, Christine M.; Evermann, James F.; Burk, Robert D.; Chen, Zigui; Mansfield, Kristin G.; Haldorson, Gary J.

In: Journal of Veterinary Diagnostic Investigation, Vol. 24, No. 4, 07.2012, p. 750-754.

Research output: Contribution to journalArticle

Rogovskyy, AS, Baszler, TV, Bradway, DS, Bruning, DL, Davitt, CM, Evermann, JF, Burk, RD, Chen, Z, Mansfield, KG & Haldorson, GJ 2012, 'A novel papillomavirus isolated from proliferative skin lesions of a wild American beaver (Castor canadensis)', Journal of Veterinary Diagnostic Investigation, vol. 24, no. 4, pp. 750-754. https://doi.org/10.1177/1040638712448654
Rogovskyy, Artem S. ; Baszler, Timothy V. ; Bradway, Daniel S. ; Bruning, Darren L. ; Davitt, Christine M. ; Evermann, James F. ; Burk, Robert D. ; Chen, Zigui ; Mansfield, Kristin G. ; Haldorson, Gary J. / A novel papillomavirus isolated from proliferative skin lesions of a wild American beaver (Castor canadensis). In: Journal of Veterinary Diagnostic Investigation. 2012 ; Vol. 24, No. 4. pp. 750-754.
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