A novel intersubunit communication mechanism in a truncated hemoglobin from Mycobacterium tuberculosis

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Abstract

HbN is a homodimeric hemoglobin from Mycobacterium tuberculosis that belongs to the newly discovered truncated hemoglobin family. The intersubunit communication mechanism in HbN was investigated by ligand replacement experiments, in which the heme-bound CO in the fully CO-saturated dimer was gradually replaced with oxygen, and vice versa. A mixed-ligand species, in which one subunit binds a CO molecule and the other subunit binds an oxygen molecule, was formed under relatively low CO concentrations. The CO-bound subunit in the mixed-ligand species was found in the open conformation, in contrast to an equilibrium mixture of open and closed conformations in the fully CO-saturated derivative. On the other hand, the O2-bound subunit stays in the closed conformation regardless of the saturation level of CO. These observations suggest that the formation of the H-bonds between the B10 tyrosine and the heme-bound dioxygen in one subunit of the dimer forces its neighboring CO-bound subunit to adopt an open conformation. The new data reported here not only reveal a novel intersubunit communication mechanism but also provide the first experimental evidence for an intermediate ligation state that is critical for a ligand-linked allosteric structural transition.

Original languageEnglish (US)
Pages (from-to)1478-1484
Number of pages7
JournalJournal of Physical Chemistry B
Volume108
Issue number4
StatePublished - Jan 29 2004

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Truncated Hemoglobins
tuberculosis
Hemoglobin
hemoglobin
Carbon Monoxide
Conformations
communication
Ligands
ligands
Communication
Dimers
dimers
Molecules
Oxygen
tyrosine
oxygen
guy wires
Heme
molecules
low concentrations

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

A novel intersubunit communication mechanism in a truncated hemoglobin from Mycobacterium tuberculosis. / Yeh, Syun-Ru.

In: Journal of Physical Chemistry B, Vol. 108, No. 4, 29.01.2004, p. 1478-1484.

Research output: Contribution to journalArticle

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