A novel inflammatory role for platelets in sickle cell disease

Jennifer Davila, Deepa Manwani, Ljiljana Vasovic, Mauro Avanzi, Joan Uehlinger, Karen Ireland, W. Beau Mitchell

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The severe pain, ischemia and organ damage that characterizes sickle cell disease (SCD) is caused by vaso-occlusion, which is the blockage of blood vessels by heterotypic aggregates of sickled erythrocytes and other cells. Vaso-occlusion is also a vasculopathy involving endothelial cell dysfunction, leukocyte activation, platelet activation and chronic inflammation resulting in the multiple adhesive interactions between cellular elements. Since platelets mediate inflammation as well as thrombosis via release of pro- and anti-inflammatory molecules, we hypothesized that platelets may play an active inflammatory role in SCD by secreting increased amounts of cytokines. Since platelets have been shown to contain mRNA and actively produce proteins, we also hypothesized that SCD platelets may contain increased cytokine mRNA. In this cross-sectional study, we sought to compare both the quantity of cytokines secreted and the cytokine mRNA content, between SCD and control platelets. We measured the secretion of Th1, Th2, and Th17-related cytokines from platelets in a cohort of SCD patients. We simultaneously measured platelet mRNA levels of those cytokines. Platelets from SCD patients secreted increased quantities of IL-1β, sCD40L, and IL-6 compared to controls. Secretion was increased in patients with alloantibodies. Additionally, mRNA of those cytokines was increased in SCD platelets. Platelets from sickle cell patients secrete increased amounts of inflammatory cytokines, and contain increased cytokine mRNA. These findings suggest a novel immunological role for platelets in SCD vasculopathy, in addition to their thrombotic role, and strengthen the rationale for the use of anti-platelet therapy in SCD.

Original languageEnglish (US)
Pages (from-to)726-729
Number of pages4
JournalPlatelets
Volume26
Issue number8
DOIs
StatePublished - Nov 17 2015

Fingerprint

Sickle Cell Anemia
Blood Platelets
Cytokines
Messenger RNA
Inflammation
Isoantibodies
Platelet Activation
Interleukin-1
Adhesives
Blood Vessels
Interleukin-6
Thrombosis
Leukocytes
Anti-Inflammatory Agents
Ischemia
Endothelial Cells
Cross-Sectional Studies
Erythrocytes

Keywords

  • Cytokines
  • inflammation
  • platelet activation
  • platelets
  • sickle cell disease

ASJC Scopus subject areas

  • Hematology

Cite this

A novel inflammatory role for platelets in sickle cell disease. / Davila, Jennifer; Manwani, Deepa; Vasovic, Ljiljana; Avanzi, Mauro; Uehlinger, Joan; Ireland, Karen; Mitchell, W. Beau.

In: Platelets, Vol. 26, No. 8, 17.11.2015, p. 726-729.

Research output: Contribution to journalArticle

Davila, Jennifer ; Manwani, Deepa ; Vasovic, Ljiljana ; Avanzi, Mauro ; Uehlinger, Joan ; Ireland, Karen ; Mitchell, W. Beau. / A novel inflammatory role for platelets in sickle cell disease. In: Platelets. 2015 ; Vol. 26, No. 8. pp. 726-729.
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