A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants

Vinayaka R. Prasad, S. P. Goff

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.

Original languageEnglish (US)
Pages (from-to)16689-16693
Number of pages5
JournalJournal of Biological Chemistry
Volume264
Issue number28
StatePublished - 1989
Externally publishedYes

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HIV Reverse Transcriptase
RNA-Directed DNA Polymerase
Bacteria
Screening

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants",
abstract = "An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.",
author = "Prasad, {Vinayaka R.} and Goff, {S. P.}",
year = "1989",
language = "English (US)",
volume = "264",
pages = "16689--16693",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "28",

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TY - JOUR

T1 - A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants

AU - Prasad, Vinayaka R.

AU - Goff, S. P.

PY - 1989

Y1 - 1989

N2 - An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.

AB - An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.

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M3 - Article

VL - 264

SP - 16689

EP - 16693

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

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