A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants

V. R. Prasad; S. P. Goff

A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants

Research output: Contribution to journal › Article › peer-review

Abstract

An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.

Original language	English (US)
Pages (from-to)	16689-16693
Number of pages	5
Journal	Journal of Biological Chemistry
Volume	264
Issue number	28
State	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants. / Prasad, V. R.; Goff, S. P.
In: Journal of Biological Chemistry, Vol. 264, No. 28, 1989, p. 16689-16693.

Research output: Contribution to journal › Article › peer-review

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abstract = "An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.",

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year = "1989",

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AU - Goff, S. P.

PY - 1989

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N2 - An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.

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JF - Journal of Biological Chemistry

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ER -

A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants

Abstract

ASJC Scopus subject areas

UN SDGs

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