A novel filtration-based processing method of liquid cytology specimens for human papillomavirus DNA testing by hybrid capture II

Philip E. Castle, Mariane Garcia-Meijide, E. Blair Holladay, Ronald Chuke, Jay Payne, Amanda Long, Heather Siefers, Franklin Demuth, Attila T. Lorincz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We evaluated a more efficient method of processing liquid-based cervical cytology specimens for human papillomavirus (HPV) DNA testing by Hybrid Capture II (HCII). Aliquots were made from 701 specimens in the following sequence: 4.0, 2.0, 1.0, 0.5, and 1.5 mL. The 4.0-mL aliquot was processed by the standard method (STP), and half of the processed material was tested by HCII. Other aliquots were processed with a new, filtration-based processing method (NPM). The 2.0-mL NPM aliquot had HCII test performance most similar to the STP, ie, similar HCII positivity (P = .4) and good test agreement (Κ = 0.85, 95% confidence interval [CI], 0.80-0.89). The 194 cytologic negatives had greater positivity by STP (P = .04) compared with the 2.0-mL aliquot processed by NPM; between-method agreement was modest (Κ = 0.54, 95% CI, 0.36-0.72). A lower positive cut point for the 2.0-mL NPM aliquot partially abrogated this minor difference. In 241 specimens diagnosed as low-grade and 31 as high-grade squamous intraepithelial lesions, there were no significant differences in HPV positivity (>85% and 90%, respectively) between STP and NPM. NPM reduces specimen handling and decreases total testing time by approximately 33% without significant losses in HCII test performance.

Original languageEnglish (US)
Pages (from-to)250-255
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume123
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

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Cell Biology
DNA
Confidence Intervals
Specimen Handling

Keywords

  • HPV
  • Human papillomavirus
  • Hybrid Capture II
  • PreservCyt

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

A novel filtration-based processing method of liquid cytology specimens for human papillomavirus DNA testing by hybrid capture II. / Castle, Philip E.; Garcia-Meijide, Mariane; Holladay, E. Blair; Chuke, Ronald; Payne, Jay; Long, Amanda; Siefers, Heather; Demuth, Franklin; Lorincz, Attila T.

In: American Journal of Clinical Pathology, Vol. 123, No. 2, 02.2005, p. 250-255.

Research output: Contribution to journalArticle

Castle, Philip E. ; Garcia-Meijide, Mariane ; Holladay, E. Blair ; Chuke, Ronald ; Payne, Jay ; Long, Amanda ; Siefers, Heather ; Demuth, Franklin ; Lorincz, Attila T. / A novel filtration-based processing method of liquid cytology specimens for human papillomavirus DNA testing by hybrid capture II. In: American Journal of Clinical Pathology. 2005 ; Vol. 123, No. 2. pp. 250-255.
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