A novel cDNA encodes a putative hRALY-like protein, hRALYL

Chao neng Ji; Jin Zhong Chen; Yi Xie; Shu Wang; Ji Qian; Enpeng Zhao; Wei Jin; Xiaozhou Wu; Wan Xiang Xu; Kang Ying; Yu Min Mao

doi:10.1023/A:1022295311177

A novel cDNA encodes a putative hRALY-like protein, hRALYL

Chao neng Ji, Jin Zhong Chen, Yi Xie, Shu Wang, Ji Qian, Enpeng Zhao, Wei Jin, Xiaozhou Wu, Wan Xiang Xu, Kang Ying, Yu Min Mao

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

High throughput cDNA sequencing and 5′-rapid amplification of cDNA ends (5′RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.20 hRALYL might be a member of hnRNPC subfamily.

Original language	English (US)
Pages (from-to)	61-67
Number of pages	7
Journal	Molecular Biology Reports
Volume	30
Issue number	1
DOIs	https://doi.org/10.1023/A:1022295311177
State	Published - Mar 2003
Externally published	Yes

Keywords

Cloning
Expression
HRALYL
HnRNP
RNA binding domain (RBD)

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1023/A:1022295311177

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@article{0712102bdfd54a02a05980536fc5ef82,

title = "A novel cDNA encodes a putative hRALY-like protein, hRALYL",

abstract = "High throughput cDNA sequencing and 5′-rapid amplification of cDNA ends (5′RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.20 hRALYL might be a member of hnRNPC subfamily.",

keywords = "Cloning, Expression, HRALYL, HnRNP, RNA binding domain (RBD)",

author = "Ji, {Chao neng} and Chen, {Jin Zhong} and Yi Xie and Shu Wang and Ji Qian and Enpeng Zhao and Wei Jin and Xiaozhou Wu and Xu, {Wan Xiang} and Kang Ying and Mao, {Yu Min}",

note = "Funding Information: We thank Professor Nolan for critical reading of the manuscript. We gratefully acknowledge financial support from the 863 project of P. R. China (grant number: 2001AA221181)",

year = "2003",

month = mar,

doi = "10.1023/A:1022295311177",

language = "English (US)",

volume = "30",

pages = "61--67",

journal = "Molecular Biology Reports",

issn = "0301-4851",

publisher = "Springer Netherlands",

number = "1",

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TY - JOUR

T1 - A novel cDNA encodes a putative hRALY-like protein, hRALYL

AU - Ji, Chao neng

AU - Chen, Jin Zhong

AU - Xie, Yi

AU - Wang, Shu

AU - Qian, Ji

AU - Zhao, Enpeng

AU - Jin, Wei

AU - Wu, Xiaozhou

AU - Xu, Wan Xiang

AU - Ying, Kang

AU - Mao, Yu Min

N1 - Funding Information: We thank Professor Nolan for critical reading of the manuscript. We gratefully acknowledge financial support from the 863 project of P. R. China (grant number: 2001AA221181)

PY - 2003/3

Y1 - 2003/3

N2 - High throughput cDNA sequencing and 5′-rapid amplification of cDNA ends (5′RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.20 hRALYL might be a member of hnRNPC subfamily.

AB - High throughput cDNA sequencing and 5′-rapid amplification of cDNA ends (5′RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.20 hRALYL might be a member of hnRNPC subfamily.

KW - Cloning

KW - Expression

KW - HRALYL

KW - HnRNP

KW - RNA binding domain (RBD)

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JO - Molecular Biology Reports

JF - Molecular Biology Reports

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A novel cDNA encodes a putative hRALY-like protein, hRALYL

Abstract

Keywords

ASJC Scopus subject areas

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