A novel cDNA encodes a putative hRALY-like protein, hRALYL

Chao neng Ji, Jin Zhong Chen, Yi Xie, Shu Wang, Ji Qian, Enpeng Zhao, Wei Jin, Xiaozhou Wu, Wan Xiang Xu, Kang Ying, Yu Min Mao

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

High throughput cDNA sequencing and 5′-rapid amplification of cDNA ends (5′RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.20 hRALYL might be a member of hnRNPC subfamily.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalMolecular Biology Reports
Volume30
Issue number1
DOIs
StatePublished - Mar 1 2003

Keywords

  • Cloning
  • Expression
  • HRALYL
  • HnRNP
  • RNA binding domain (RBD)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'A novel cDNA encodes a putative hRALY-like protein, hRALYL'. Together they form a unique fingerprint.

  • Cite this

    Ji, C. N., Chen, J. Z., Xie, Y., Wang, S., Qian, J., Zhao, E., Jin, W., Wu, X., Xu, W. X., Ying, K., & Mao, Y. M. (2003). A novel cDNA encodes a putative hRALY-like protein, hRALYL. Molecular Biology Reports, 30(1), 61-67. https://doi.org/10.1023/A:1022295311177