A novel cDNA encodes a putative hRALY-like protein, hRALYL

Chao neng Ji, Jin Zhong Chen, Yi Xie, Shu Wang, Ji Qian, Enpeng Zhao, Wei Jin, Xiaozhou Wu, Wan Xiang Xu, Kang Ying, Yu Min Mao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

High throughput cDNA sequencing and 5′-rapid amplification of cDNA ends (5′RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.20 hRALYL might be a member of hnRNPC subfamily.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalMolecular Biology Reports
Volume30
Issue number1
DOIs
StatePublished - Mar 2003
Externally publishedYes

Fingerprint

Complementary DNA
Proteins
Reading
Brain
Genes
Human Chromosomes
Chromosomes
Northern Blotting
Amplification
Exons
Throughput
Tissue
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cloning
  • Expression
  • HnRNP
  • HRALYL
  • RNA binding domain (RBD)

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

Cite this

Ji, C. N., Chen, J. Z., Xie, Y., Wang, S., Qian, J., Zhao, E., ... Mao, Y. M. (2003). A novel cDNA encodes a putative hRALY-like protein, hRALYL. Molecular Biology Reports, 30(1), 61-67. https://doi.org/10.1023/A:1022295311177

A novel cDNA encodes a putative hRALY-like protein, hRALYL. / Ji, Chao neng; Chen, Jin Zhong; Xie, Yi; Wang, Shu; Qian, Ji; Zhao, Enpeng; Jin, Wei; Wu, Xiaozhou; Xu, Wan Xiang; Ying, Kang; Mao, Yu Min.

In: Molecular Biology Reports, Vol. 30, No. 1, 03.2003, p. 61-67.

Research output: Contribution to journalArticle

Ji, CN, Chen, JZ, Xie, Y, Wang, S, Qian, J, Zhao, E, Jin, W, Wu, X, Xu, WX, Ying, K & Mao, YM 2003, 'A novel cDNA encodes a putative hRALY-like protein, hRALYL', Molecular Biology Reports, vol. 30, no. 1, pp. 61-67. https://doi.org/10.1023/A:1022295311177
Ji, Chao neng ; Chen, Jin Zhong ; Xie, Yi ; Wang, Shu ; Qian, Ji ; Zhao, Enpeng ; Jin, Wei ; Wu, Xiaozhou ; Xu, Wan Xiang ; Ying, Kang ; Mao, Yu Min. / A novel cDNA encodes a putative hRALY-like protein, hRALYL. In: Molecular Biology Reports. 2003 ; Vol. 30, No. 1. pp. 61-67.
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AU - Qian, Ji

AU - Zhao, Enpeng

AU - Jin, Wei

AU - Wu, Xiaozhou

AU - Xu, Wan Xiang

AU - Ying, Kang

AU - Mao, Yu Min

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AB - High throughput cDNA sequencing and 5′-rapid amplification of cDNA ends (5′RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.20 hRALYL might be a member of hnRNPC subfamily.

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