A novel addition to the T cell repertory

Cell surface expression of tumor necrosis factor/cachectin by activated normal human T cells

Milan Kinkhabwala, P. Sehajpal, E. Skolnik, D. Smith, V. K. Sharma, H. Vlassara, A. Cerami, M. Suthanthiran

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Expression of the pluripotent molecule TNF in a focused and antigen-restricted fashion might provide an advantage to the host organism. Given the central role of T cells in antigen-specific immunity, we examined whether activated T cells express TNF on their cell surface. FACS analysis of highly purified normal human T cells labeled with an anti-TNF mAb revealed that T cells express cell surface TNF when signaled with the synergistic combination of a calcium ionophore, ionomycin, and a protein kinase C activator, 12-o-tetradecanoyl phorbol acetate. Cell surface radioiodination studies of stimulated T cells demonstrated the presence of 26-kD transmembrane protein, a size predicted by TNF cDNA and different from that of the 17-kD secreted TNF molecule. The induced cell surface expression of TNF could be blocked with cyclosporine and/or methylprednisolone, and Northern analysis for TNF-specific transcripts revealed that this inhibitory effect occurs pretranslationally. Our demonstration for the first time that stimulated normal human T cells display cell surface TNF provides a mechanistic basis for the realization of effects of TNF in an antigen-specific fashion.

Original languageEnglish (US)
Pages (from-to)941-946
Number of pages6
JournalJournal of Experimental Medicine
Volume171
Issue number3
StatePublished - Mar 1 1990
Externally publishedYes

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Tumor Necrosis Factor-alpha
T-Lymphocytes
Antigens
Ionomycin
Calcium Ionophores
Methylprednisolone
Protein Kinase C
Cyclosporine
Immunity
Acetates
Complementary DNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

A novel addition to the T cell repertory : Cell surface expression of tumor necrosis factor/cachectin by activated normal human T cells. / Kinkhabwala, Milan; Sehajpal, P.; Skolnik, E.; Smith, D.; Sharma, V. K.; Vlassara, H.; Cerami, A.; Suthanthiran, M.

In: Journal of Experimental Medicine, Vol. 171, No. 3, 01.03.1990, p. 941-946.

Research output: Contribution to journalArticle

Kinkhabwala, M, Sehajpal, P, Skolnik, E, Smith, D, Sharma, VK, Vlassara, H, Cerami, A & Suthanthiran, M 1990, 'A novel addition to the T cell repertory: Cell surface expression of tumor necrosis factor/cachectin by activated normal human T cells', Journal of Experimental Medicine, vol. 171, no. 3, pp. 941-946.
Kinkhabwala, Milan ; Sehajpal, P. ; Skolnik, E. ; Smith, D. ; Sharma, V. K. ; Vlassara, H. ; Cerami, A. ; Suthanthiran, M. / A novel addition to the T cell repertory : Cell surface expression of tumor necrosis factor/cachectin by activated normal human T cells. In: Journal of Experimental Medicine. 1990 ; Vol. 171, No. 3. pp. 941-946.
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