Abstract
Expression of the pluripotent molecule TNF in a focused and antigen-restricted fashion might provide an advantage to the host organism. Given the central role of T cells in antigen-specific immunity, we examined whether activated T cells express TNF on their cell surface. FACS analysis of highly purified normal human T cells labeled with an anti-TNF mAb revealed that T cells express cell surface TNF when signaled with the synergistic combination of a calcium ionophore, ionomycin, and a protein kinase C activator, 12-o-tetradecanoyl phorbol acetate. Cell surface radioiodination studies of stimulated T cells demonstrated the presence of 26-kD transmembrane protein, a size predicted by TNF cDNA and different from that of the 17-kD secreted TNF molecule. The induced cell surface expression of TNF could be blocked with cyclosporine and/or methylprednisolone, and Northern analysis for TNF-specific transcripts revealed that this inhibitory effect occurs pretranslationally. Our demonstration for the first time that stimulated normal human T cells display cell surface TNF provides a mechanistic basis for the realization of effects of TNF in an antigen-specific fashion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 941-946 |
| Number of pages | 6 |
| Journal | Journal of Experimental Medicine |
| Volume | 171 |
| Issue number | 3 |
| State | Published - Mar 1 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
A novel addition to the T cell repertory : Cell surface expression of tumor necrosis factor/cachectin by activated normal human T cells. / Kinkhabwala, Milan; Sehajpal, P.; Skolnik, E.; Smith, D.; Sharma, V. K.; Vlassara, H.; Cerami, A.; Suthanthiran, M.
In: Journal of Experimental Medicine, Vol. 171, No. 3, 01.03.1990, p. 941-946.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel addition to the T cell repertory
T2 - Cell surface expression of tumor necrosis factor/cachectin by activated normal human T cells
AU - Kinkhabwala, Milan
AU - Sehajpal, P.
AU - Skolnik, E.
AU - Smith, D.
AU - Sharma, V. K.
AU - Vlassara, H.
AU - Cerami, A.
AU - Suthanthiran, M.
PY - 1990/3/1
Y1 - 1990/3/1
N2 - Expression of the pluripotent molecule TNF in a focused and antigen-restricted fashion might provide an advantage to the host organism. Given the central role of T cells in antigen-specific immunity, we examined whether activated T cells express TNF on their cell surface. FACS analysis of highly purified normal human T cells labeled with an anti-TNF mAb revealed that T cells express cell surface TNF when signaled with the synergistic combination of a calcium ionophore, ionomycin, and a protein kinase C activator, 12-o-tetradecanoyl phorbol acetate. Cell surface radioiodination studies of stimulated T cells demonstrated the presence of 26-kD transmembrane protein, a size predicted by TNF cDNA and different from that of the 17-kD secreted TNF molecule. The induced cell surface expression of TNF could be blocked with cyclosporine and/or methylprednisolone, and Northern analysis for TNF-specific transcripts revealed that this inhibitory effect occurs pretranslationally. Our demonstration for the first time that stimulated normal human T cells display cell surface TNF provides a mechanistic basis for the realization of effects of TNF in an antigen-specific fashion.
AB - Expression of the pluripotent molecule TNF in a focused and antigen-restricted fashion might provide an advantage to the host organism. Given the central role of T cells in antigen-specific immunity, we examined whether activated T cells express TNF on their cell surface. FACS analysis of highly purified normal human T cells labeled with an anti-TNF mAb revealed that T cells express cell surface TNF when signaled with the synergistic combination of a calcium ionophore, ionomycin, and a protein kinase C activator, 12-o-tetradecanoyl phorbol acetate. Cell surface radioiodination studies of stimulated T cells demonstrated the presence of 26-kD transmembrane protein, a size predicted by TNF cDNA and different from that of the 17-kD secreted TNF molecule. The induced cell surface expression of TNF could be blocked with cyclosporine and/or methylprednisolone, and Northern analysis for TNF-specific transcripts revealed that this inhibitory effect occurs pretranslationally. Our demonstration for the first time that stimulated normal human T cells display cell surface TNF provides a mechanistic basis for the realization of effects of TNF in an antigen-specific fashion.
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M3 - Article
VL - 171
SP - 941
EP - 946
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 3
ER -