A Note of Caution: Variable Cytokeratin Staining in Sentinel Node Metastases

Jennifer Zeng; Melissa Ann Alexander; Diana Nimeh; Farbod Darvishian

doi:10.1177/1066896915596811

A Note of Caution: Variable Cytokeratin Staining in Sentinel Node Metastases

Jennifer Zeng, Melissa Ann Alexander, Diana Nimeh, Farbod Darvishian

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Sentinel lymph node biopsy is the current standard procedure used to stage patients with breast cancer. The best histological method in evaluating sentinel nodes is highly debated among institutions and is thus not standardized. The optimal histological analysis is a balance between comprehensive evaluation of the sentinel nodes and cost effectiveness. One commonly used approach is serial sectioning and alternately staining with hemotoxylin and eosin and AE1/AE3 cytokeratin immunohistochemistry analysis. We report 2 cases of metastatic carcinoma demonstrating negative staining for AE1/AE3. This observation highlights a rare but potential pitfall to this commonly used strategy in assessing sentinel lymph node biopsies in breast cancer.

Original language	English (US)
Pages (from-to)	549-552
Number of pages	4
Journal	International Journal of Surgical Pathology
Volume	23
Issue number	7
DOIs	https://doi.org/10.1177/1066896915596811
State	Published - Oct 3 2015
Externally published	Yes

Keywords

breast
cytokeratin
sentinel lymph node

ASJC Scopus subject areas

Surgery
Anatomy
Pathology and Forensic Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/1066896915596811

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title = "A Note of Caution: Variable Cytokeratin Staining in Sentinel Node Metastases",

abstract = "Sentinel lymph node biopsy is the current standard procedure used to stage patients with breast cancer. The best histological method in evaluating sentinel nodes is highly debated among institutions and is thus not standardized. The optimal histological analysis is a balance between comprehensive evaluation of the sentinel nodes and cost effectiveness. One commonly used approach is serial sectioning and alternately staining with hemotoxylin and eosin and AE1/AE3 cytokeratin immunohistochemistry analysis. We report 2 cases of metastatic carcinoma demonstrating negative staining for AE1/AE3. This observation highlights a rare but potential pitfall to this commonly used strategy in assessing sentinel lymph node biopsies in breast cancer.",

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year = "2015",

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T1 - A Note of Caution

T2 - Variable Cytokeratin Staining in Sentinel Node Metastases

AU - Zeng, Jennifer

AU - Alexander, Melissa Ann

AU - Nimeh, Diana

AU - Darvishian, Farbod

N1 - Publisher Copyright: © The Author(s) 2015.

PY - 2015/10/3

Y1 - 2015/10/3

N2 - Sentinel lymph node biopsy is the current standard procedure used to stage patients with breast cancer. The best histological method in evaluating sentinel nodes is highly debated among institutions and is thus not standardized. The optimal histological analysis is a balance between comprehensive evaluation of the sentinel nodes and cost effectiveness. One commonly used approach is serial sectioning and alternately staining with hemotoxylin and eosin and AE1/AE3 cytokeratin immunohistochemistry analysis. We report 2 cases of metastatic carcinoma demonstrating negative staining for AE1/AE3. This observation highlights a rare but potential pitfall to this commonly used strategy in assessing sentinel lymph node biopsies in breast cancer.

AB - Sentinel lymph node biopsy is the current standard procedure used to stage patients with breast cancer. The best histological method in evaluating sentinel nodes is highly debated among institutions and is thus not standardized. The optimal histological analysis is a balance between comprehensive evaluation of the sentinel nodes and cost effectiveness. One commonly used approach is serial sectioning and alternately staining with hemotoxylin and eosin and AE1/AE3 cytokeratin immunohistochemistry analysis. We report 2 cases of metastatic carcinoma demonstrating negative staining for AE1/AE3. This observation highlights a rare but potential pitfall to this commonly used strategy in assessing sentinel lymph node biopsies in breast cancer.

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KW - cytokeratin

KW - sentinel lymph node

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A Note of Caution: Variable Cytokeratin Staining in Sentinel Node Metastases

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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