A new physiological role of copper amine oxidases: Cardioprotection against reactive oxygen intermediates

M. A. Mateescu, M. J. Dumoulin, X. T. Wang, R. Nadeau, B. Mondovi

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The antioxidant effects of serum amine oxidase (SAO) against electrolysis induced oxygen free radicals (̇O2-,̇OH, 1O2) and their by-products (H2, O2, HOCl) were determined in vitro by the DPD (N,N-diethyl-p-phenylenediamine) colorimetric method. SAO (1 μM) displayed a high antioxidant capacity (91%), comparable to that of ceruloplasmine (89%), and higher to that of superoxide dismutase (SOD) in equimolar concentrations (38%). Cardioprotective effects of SAO against oxidative injury, were determined ex vivo on isolated rat hearts (Langendorff), exposed to oxygen free radicals (OFR) generated by electrolysis (10 mA, 1 min) in the absence and presence of different concentrations (0-0.5 μM) of SAO in the Krebs-Henseleit perfusion solutions. Electrocardiogram and left ventricular pressure (LVP), in the non-treated hearts, were altered by OFR formation. In the presence of SAO, a concentration dependent reduction of these alterations was observed. The protective effect afforded by 0.5 μM SAO (cardioprotection of 94%) was slightly higher than that of 1 μM ceruloplasmin (83-89%) and higher than that of 1 μM SOD (20-45%). With BSA, no cardioprotection at all was observed. SAO, a circulating extracellular enzyme for most of mammalians, appears as an effective cardioprotective agent against myocardial injuries generated by OFR.

Original languageEnglish (US)
Pages (from-to)110-121
Number of pages12
JournalJournal of Physiology and Pharmacology
Volume48
Issue numberSUPPL. 2
StatePublished - Dec 1 1997

Keywords

  • Antioxidant
  • Free radical scavenger
  • Isolated heart
  • Oxygen free radicals
  • Reactive oxygen intermediates
  • Serum amine oxidase

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

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    Mateescu, M. A., Dumoulin, M. J., Wang, X. T., Nadeau, R., & Mondovi, B. (1997). A new physiological role of copper amine oxidases: Cardioprotection against reactive oxygen intermediates. Journal of Physiology and Pharmacology, 48(SUPPL. 2), 110-121.