A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer

Fabien Kieken; Françoise Paquet; Fabienne Brulé; Jacques Paoletti; Gérard Lancelot

doi:10.1093/nar/gkj427

A new NMR solution structure of the SL1 HIV-1_Lai loop-loop dimer

Fabien Kieken, Françoise Paquet, Fabienne Brulé, Jacques Paoletti, Gérard Lancelot

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Dimerization of genomic RNA is directly related with the event of encapsidation and maturation of the virion. The initiating sequence of the dimerization is a short autocomplementary region in the hairpin loop SL1. We describe here a new solution structure of the RNA dimerization initiation site (DIS) of HIV-1_Lai. NMR pulsed field-gradient spin-echo techniques and multidimensional heteronuclear NMR spectroscopy indicate that this structure is formed by two hairpins linked by six Watson-Crick GC base pairs. Hinges between the stems and the loops are stabilized by intra and intermolecular interactions involving the A8, A9 and A16 adenines. The coaxial alignment of the three A-type helices present in the structure is supported by previous crystallography analysis but the A8 and A9 adenines are found in a bulged in position. These data suggest the existence of an equilibrium between bulged in and bulged out conformations in solution.

Original language	English (US)
Pages (from-to)	343-352
Number of pages	10
Journal	Nucleic acids research
Volume	34
Issue number	1
DOIs	https://doi.org/10.1093/nar/gkj427
State	Published - Jan 2006
Externally published	Yes

ASJC Scopus subject areas

Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/nar/gkj427

Cite this

@article{aeb3bb20644b4dd8b7d2a8cd3851a903,

title = "A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer",

abstract = "Dimerization of genomic RNA is directly related with the event of encapsidation and maturation of the virion. The initiating sequence of the dimerization is a short autocomplementary region in the hairpin loop SL1. We describe here a new solution structure of the RNA dimerization initiation site (DIS) of HIV-1Lai. NMR pulsed field-gradient spin-echo techniques and multidimensional heteronuclear NMR spectroscopy indicate that this structure is formed by two hairpins linked by six Watson-Crick GC base pairs. Hinges between the stems and the loops are stabilized by intra and intermolecular interactions involving the A8, A9 and A16 adenines. The coaxial alignment of the three A-type helices present in the structure is supported by previous crystallography analysis but the A8 and A9 adenines are found in a bulged in position. These data suggest the existence of an equilibrium between bulged in and bulged out conformations in solution.",

author = "Fabien Kieken and Fran{\c c}oise Paquet and Fabienne Brul{\'e} and Jacques Paoletti and G{\'e}rard Lancelot",

note = "Funding Information: The authors thank the Laboratoire de RMN, Institut de Biologie Structurale in Grenoble, France, for running NMR spectra at 800 MHz. This work was supported by Agence Nationale de la Recherche sur le Sida grant (J.P.). F.K. was supported by a fellowship from the Minist{\`e}re Franc¸ais de l{\textquoteright}Education Nationale, de la Recherche et de la Technologie. The Open Access publication charges for this article were waived by Oxford University Press.",

year = "2006",

month = jan,

doi = "10.1093/nar/gkj427",

language = "English (US)",

volume = "34",

pages = "343--352",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer

AU - Kieken, Fabien

AU - Paquet, Françoise

AU - Brulé, Fabienne

AU - Paoletti, Jacques

AU - Lancelot, Gérard

N1 - Funding Information: The authors thank the Laboratoire de RMN, Institut de Biologie Structurale in Grenoble, France, for running NMR spectra at 800 MHz. This work was supported by Agence Nationale de la Recherche sur le Sida grant (J.P.). F.K. was supported by a fellowship from the Ministère Franc¸ais de l’Education Nationale, de la Recherche et de la Technologie. The Open Access publication charges for this article were waived by Oxford University Press.

PY - 2006/1

Y1 - 2006/1

N2 - Dimerization of genomic RNA is directly related with the event of encapsidation and maturation of the virion. The initiating sequence of the dimerization is a short autocomplementary region in the hairpin loop SL1. We describe here a new solution structure of the RNA dimerization initiation site (DIS) of HIV-1Lai. NMR pulsed field-gradient spin-echo techniques and multidimensional heteronuclear NMR spectroscopy indicate that this structure is formed by two hairpins linked by six Watson-Crick GC base pairs. Hinges between the stems and the loops are stabilized by intra and intermolecular interactions involving the A8, A9 and A16 adenines. The coaxial alignment of the three A-type helices present in the structure is supported by previous crystallography analysis but the A8 and A9 adenines are found in a bulged in position. These data suggest the existence of an equilibrium between bulged in and bulged out conformations in solution.

AB - Dimerization of genomic RNA is directly related with the event of encapsidation and maturation of the virion. The initiating sequence of the dimerization is a short autocomplementary region in the hairpin loop SL1. We describe here a new solution structure of the RNA dimerization initiation site (DIS) of HIV-1Lai. NMR pulsed field-gradient spin-echo techniques and multidimensional heteronuclear NMR spectroscopy indicate that this structure is formed by two hairpins linked by six Watson-Crick GC base pairs. Hinges between the stems and the loops are stabilized by intra and intermolecular interactions involving the A8, A9 and A16 adenines. The coaxial alignment of the three A-type helices present in the structure is supported by previous crystallography analysis but the A8 and A9 adenines are found in a bulged in position. These data suggest the existence of an equilibrium between bulged in and bulged out conformations in solution.

UR - http://www.scopus.com/inward/record.url?scp=31544475991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31544475991&partnerID=8YFLogxK

U2 - 10.1093/nar/gkj427

DO - 10.1093/nar/gkj427

M3 - Article

C2 - 16410614

AN - SCOPUS:31544475991

SN - 0305-1048

VL - 34

SP - 343

EP - 352

JO - Nucleic acids research

JF - Nucleic acids research

IS - 1

ER -