A new mouse model for evaluating the immunotherapy of human colorectal cancer

Heidi Hörig; Alberto Wainstein; Li Long; Howard L. Kaufman; Doron Kahn; Sandeep Soni; Akiva Marcus; Howard L. Kaufman; Winfried Edelmann; Raju Kucherlapati

A new mouse model for evaluating the immunotherapy of human colorectal cancer

Heidi Hörig, Alberto Wainstein, Li Long, Howard L. Kaufman, Doron Kahn, Sandeep Soni, Akiva Marcus, Howard L. Kaufman, Winfried Edelmann, Raju Kucherlapati

Cell Biology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A new murine model of human colorectal cancer was generated by crossing human carcinoembryonic antigen (CEA) transgenic mice (H2K^b) with adenomatous polyposis coli (Apc1638N) knockout mice (H2K^b). The resulting hybrid mice developed gastrointestinal polyps in 6-8 months that progressed to invasive carcinomas with a similar pattern of dysplasia and CEA expression as observed in human colorectal cancer. These animals exhibited incomplete or partial tolerance to CEA as evidenced by delayed growth of CEA-expressing tumors and the inability to inhibit CEA-specific CTL responses. These results have important implications for understanding the role of CEA-specific immunity in human colon cancer patients and suggest that vaccine strategies targeting CEA may be feasible. This model provides a powerful system for evaluating antigen-specific tumor immunity against spontaneous tumors arising in an orthotopic location and permits evaluation of therapeutic vaccine strategies for human colorectal cancer.

Original language	English (US)
Pages (from-to)	8520-8526
Number of pages	7
Journal	Cancer research
Volume	61
Issue number	23
State	Published - Dec 1 2001

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "A new mouse model for evaluating the immunotherapy of human colorectal cancer",

abstract = "A new murine model of human colorectal cancer was generated by crossing human carcinoembryonic antigen (CEA) transgenic mice (H2Kb) with adenomatous polyposis coli (Apc1638N) knockout mice (H2Kb). The resulting hybrid mice developed gastrointestinal polyps in 6-8 months that progressed to invasive carcinomas with a similar pattern of dysplasia and CEA expression as observed in human colorectal cancer. These animals exhibited incomplete or partial tolerance to CEA as evidenced by delayed growth of CEA-expressing tumors and the inability to inhibit CEA-specific CTL responses. These results have important implications for understanding the role of CEA-specific immunity in human colon cancer patients and suggest that vaccine strategies targeting CEA may be feasible. This model provides a powerful system for evaluating antigen-specific tumor immunity against spontaneous tumors arising in an orthotopic location and permits evaluation of therapeutic vaccine strategies for human colorectal cancer.",

author = "Heidi H{\"o}rig and Alberto Wainstein and Li Long and Kaufman, {Howard L.} and Doron Kahn and Sandeep Soni and Akiva Marcus and Kaufman, {Howard L.} and Winfried Edelmann and Raju Kucherlapati",

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AU - Hörig, Heidi

AU - Wainstein, Alberto

AU - Long, Li

AU - Kaufman, Howard L.

AU - Kahn, Doron

AU - Soni, Sandeep

AU - Marcus, Akiva

AU - Kaufman, Howard L.

AU - Edelmann, Winfried

AU - Kucherlapati, Raju

PY - 2001/12/1

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N2 - A new murine model of human colorectal cancer was generated by crossing human carcinoembryonic antigen (CEA) transgenic mice (H2Kb) with adenomatous polyposis coli (Apc1638N) knockout mice (H2Kb). The resulting hybrid mice developed gastrointestinal polyps in 6-8 months that progressed to invasive carcinomas with a similar pattern of dysplasia and CEA expression as observed in human colorectal cancer. These animals exhibited incomplete or partial tolerance to CEA as evidenced by delayed growth of CEA-expressing tumors and the inability to inhibit CEA-specific CTL responses. These results have important implications for understanding the role of CEA-specific immunity in human colon cancer patients and suggest that vaccine strategies targeting CEA may be feasible. This model provides a powerful system for evaluating antigen-specific tumor immunity against spontaneous tumors arising in an orthotopic location and permits evaluation of therapeutic vaccine strategies for human colorectal cancer.

AB - A new murine model of human colorectal cancer was generated by crossing human carcinoembryonic antigen (CEA) transgenic mice (H2Kb) with adenomatous polyposis coli (Apc1638N) knockout mice (H2Kb). The resulting hybrid mice developed gastrointestinal polyps in 6-8 months that progressed to invasive carcinomas with a similar pattern of dysplasia and CEA expression as observed in human colorectal cancer. These animals exhibited incomplete or partial tolerance to CEA as evidenced by delayed growth of CEA-expressing tumors and the inability to inhibit CEA-specific CTL responses. These results have important implications for understanding the role of CEA-specific immunity in human colon cancer patients and suggest that vaccine strategies targeting CEA may be feasible. This model provides a powerful system for evaluating antigen-specific tumor immunity against spontaneous tumors arising in an orthotopic location and permits evaluation of therapeutic vaccine strategies for human colorectal cancer.

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A new mouse model for evaluating the immunotherapy of human colorectal cancer

Abstract

ASJC Scopus subject areas

UN SDGs

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