A new iron-binding protein isolated from intestinal mucosa

Simeon Pollack, Fred D. Lasky

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A new iron-binding protein contained in guinea pig intestinal mucosa has been purified to homogeneity. The protein has a molecular weight of 78,000 in a nondissociating system and 43,000 in SDS-gel electrophoresis. It binds approximately 2 moles of iron per mole with a formation constant of 1019 at pH 7. It is distinguished from transferrin and lactoferrin by differences on DEAE-Sephadex chromatography and spectroscopy and by its failure to cross-react with antisera to these other iron-binding proteins.

Original languageEnglish (US)
Pages (from-to)670-679
Number of pages10
JournalThe Journal of Laboratory and Clinical Medicine
Volume87
Issue number4
StatePublished - 1976

Fingerprint

Iron-Binding Proteins
Intestinal Mucosa
DEAE-Dextran
Lactoferrin
Transferrin
Chromatography
Electrophoresis
Immune Sera
Spectrum Analysis
Guinea Pigs
Iron
Molecular Weight
Gels
Molecular weight
Spectroscopy
Proteins
Mucous Membrane

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

A new iron-binding protein isolated from intestinal mucosa. / Pollack, Simeon; Lasky, Fred D.

In: The Journal of Laboratory and Clinical Medicine, Vol. 87, No. 4, 1976, p. 670-679.

Research output: Contribution to journalArticle

@article{8b97e8d1ed774c1e99a13fddab0fb299,
title = "A new iron-binding protein isolated from intestinal mucosa",
abstract = "A new iron-binding protein contained in guinea pig intestinal mucosa has been purified to homogeneity. The protein has a molecular weight of 78,000 in a nondissociating system and 43,000 in SDS-gel electrophoresis. It binds approximately 2 moles of iron per mole with a formation constant of 1019 at pH 7. It is distinguished from transferrin and lactoferrin by differences on DEAE-Sephadex chromatography and spectroscopy and by its failure to cross-react with antisera to these other iron-binding proteins.",
author = "Simeon Pollack and Lasky, {Fred D.}",
year = "1976",
language = "English (US)",
volume = "87",
pages = "670--679",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - A new iron-binding protein isolated from intestinal mucosa

AU - Pollack, Simeon

AU - Lasky, Fred D.

PY - 1976

Y1 - 1976

N2 - A new iron-binding protein contained in guinea pig intestinal mucosa has been purified to homogeneity. The protein has a molecular weight of 78,000 in a nondissociating system and 43,000 in SDS-gel electrophoresis. It binds approximately 2 moles of iron per mole with a formation constant of 1019 at pH 7. It is distinguished from transferrin and lactoferrin by differences on DEAE-Sephadex chromatography and spectroscopy and by its failure to cross-react with antisera to these other iron-binding proteins.

AB - A new iron-binding protein contained in guinea pig intestinal mucosa has been purified to homogeneity. The protein has a molecular weight of 78,000 in a nondissociating system and 43,000 in SDS-gel electrophoresis. It binds approximately 2 moles of iron per mole with a formation constant of 1019 at pH 7. It is distinguished from transferrin and lactoferrin by differences on DEAE-Sephadex chromatography and spectroscopy and by its failure to cross-react with antisera to these other iron-binding proteins.

UR - http://www.scopus.com/inward/record.url?scp=0017250221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017250221&partnerID=8YFLogxK

M3 - Article

VL - 87

SP - 670

EP - 679

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 4

ER -