A new iron-binding protein contained in guinea pig intestinal mucosa has been purified to homogeneity. The protein has a molecular weight of 78,000 in a nondissociating system and 43,000 in SDS-gel electrophoresis. It binds approximately 2 moles of iron per mole with a formation constant of 1019 at pH 7. It is distinguished from transferrin and lactoferrin by differences on DEAE-Sephadex chromatography and spectroscopy and by its failure to cross-react with antisera to these other iron-binding proteins.
|Original language||English (US)|
|Number of pages||10|
|Journal||The Journal of Laboratory and Clinical Medicine|
|Publication status||Published - Apr 1976|
ASJC Scopus subject areas
- Pathology and Forensic Medicine