A new donor cornea harvesting technique for posterior lamellar keratoplasty

O. Suwan-Apichon, M. Rizen, J. M G Reyes, S. Herretes, A. Behrens, W. J. Stark, Roy S. Chuck

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aims: To describe a technique for posterior lamellar keratoplasty donor preparation. Methods: In an experimental study eight human donor research corneas were mounted onto an artificial anterior chamber and deep stromal pockets dissected. Four corneas were mounted in the standard endothelial side down orientation and dissected using standard instruments (group 1). Another four corneas were mounted endothelial side up and dissected using a flat spatula (group 2). Trephined lamellar graft thickness was assessed by ultrasound pachymetry. The grafts were also analysed using vital staining of the endothelium and standard histological preparation. Results: Achieved posterior graft thickness was 118 (SD 32) μm (group 1) and 92 (23) μm (group 2) (p = 0.324). Percentage of devitalised endothelial cells was 0.86% (1.48%) (group 1) and 3.9% (2.9%) (group 2) (p = 0.185). The dissections using both harvesting techniques remained in plane and were smooth. Conclusions: A blunt spatula and endothelium side up orientation on an artificial anterior chamber can be used to create posterior lamellar dissections without compromising endothelial cell number or planarity when compared to standard endothelium side down harvest.

Original languageEnglish (US)
Pages (from-to)1100-1101
Number of pages2
JournalBritish Journal of Ophthalmology
Volume89
Issue number9
DOIs
StatePublished - Sep 2005
Externally publishedYes

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Corneal Transplantation
Cornea
Endothelium
Anterior Chamber
Transplants
Dissection
Endothelial Cells
Cell Count
Staining and Labeling
Research

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Suwan-Apichon, O., Rizen, M., Reyes, J. M. G., Herretes, S., Behrens, A., Stark, W. J., & Chuck, R. S. (2005). A new donor cornea harvesting technique for posterior lamellar keratoplasty. British Journal of Ophthalmology, 89(9), 1100-1101. https://doi.org/10.1136/bjo.2004.064337

A new donor cornea harvesting technique for posterior lamellar keratoplasty. / Suwan-Apichon, O.; Rizen, M.; Reyes, J. M G; Herretes, S.; Behrens, A.; Stark, W. J.; Chuck, Roy S.

In: British Journal of Ophthalmology, Vol. 89, No. 9, 09.2005, p. 1100-1101.

Research output: Contribution to journalArticle

Suwan-Apichon, O, Rizen, M, Reyes, JMG, Herretes, S, Behrens, A, Stark, WJ & Chuck, RS 2005, 'A new donor cornea harvesting technique for posterior lamellar keratoplasty', British Journal of Ophthalmology, vol. 89, no. 9, pp. 1100-1101. https://doi.org/10.1136/bjo.2004.064337
Suwan-Apichon O, Rizen M, Reyes JMG, Herretes S, Behrens A, Stark WJ et al. A new donor cornea harvesting technique for posterior lamellar keratoplasty. British Journal of Ophthalmology. 2005 Sep;89(9):1100-1101. https://doi.org/10.1136/bjo.2004.064337
Suwan-Apichon, O. ; Rizen, M. ; Reyes, J. M G ; Herretes, S. ; Behrens, A. ; Stark, W. J. ; Chuck, Roy S. / A new donor cornea harvesting technique for posterior lamellar keratoplasty. In: British Journal of Ophthalmology. 2005 ; Vol. 89, No. 9. pp. 1100-1101.
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