A new diagnostic for cancer dynamics

Status and initial tests of the NANIVID

Waseem K. Raja, Bojana Gligorijevic, John S. Condeelis, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

The Tumor MicroEnvironment for Metastasis (TMEM) is a critical determinant which will presage the evolution of primary tumors and the resulting metastatic dynamics. Primary tumor cells up and down regulate certain genes which increase motility and cause a disregard for positional information. We report on the development of a new tool for the documentation of cancer cell migration (initial targets: the rat mammary adenocarcinoma cell lines MTLn3 with an over expression of Mena+++). This tool, the NANo IntraVital Device (NANIVID), is a multi-functional nanosystem composed of a chemoattractant source (hydrogel-EGF), capsule (cell trap), counter (transparent, interdigitated electrode arrays for sensing cell arrival), and remote reporter (readout electronics). The device will be retrieved from the tumor site and the cells will be expelled for subsequent assay. The NANIVID will be used in conjunction with the current catheter-based approach in which a needle is loaded with a chemoattractant source and injected into the tumor. A major drawback in the catheter approach is the short cell collection time and lack of real time registering and reporting of cell arrival. This paper will present the current status of the NANIVID prototypes developed in which a transparent implantable device is loaded with chemoattractant source and placed near candidate mammary gland tumors in an established rat model for multiple days or weeks. This series of experiments will allow the comparison of methods and to benchmark the NANIVID for use in research. Initial results of these experiments and NANIVID design modifications will be presented.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7207
DOIs
StatePublished - 2009
EventMicrofluidics, BioMEMS, and Medical Microsystems VII - San Jose, CA, United States
Duration: Jan 26 2009Jan 28 2009

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems VII
CountryUnited States
CitySan Jose, CA
Period1/26/091/28/09

Fingerprint

Tumors
cancer
tumors
Equipment and Supplies
Chemotactic Factors
Neoplasms
Catheters
Cells
cells
Remote readouts
Rats
rats
arrivals
Nanosystems
Equipment Design
mammary glands
Benchmarking
Tumor Microenvironment
Hydrogel
Human Mammary Glands

Keywords

  • Controlled release
  • ELISA
  • Epidermal growth factor (EGF)
  • Hydrogel
  • Indium doped tin oxide electrodes
  • Poly (ethylene glycol) DiAcrylate (PEGDA)
  • Tumor cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Raja, W. K., Gligorijevic, B., Condeelis, J. S., & Castracane, J. (2009). A new diagnostic for cancer dynamics: Status and initial tests of the NANIVID. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7207). [72070E] https://doi.org/10.1117/12.809366

A new diagnostic for cancer dynamics : Status and initial tests of the NANIVID. / Raja, Waseem K.; Gligorijevic, Bojana; Condeelis, John S.; Castracane, James.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7207 2009. 72070E.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Raja, WK, Gligorijevic, B, Condeelis, JS & Castracane, J 2009, A new diagnostic for cancer dynamics: Status and initial tests of the NANIVID. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7207, 72070E, Microfluidics, BioMEMS, and Medical Microsystems VII, San Jose, CA, United States, 1/26/09. https://doi.org/10.1117/12.809366
Raja WK, Gligorijevic B, Condeelis JS, Castracane J. A new diagnostic for cancer dynamics: Status and initial tests of the NANIVID. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7207. 2009. 72070E https://doi.org/10.1117/12.809366
Raja, Waseem K. ; Gligorijevic, Bojana ; Condeelis, John S. ; Castracane, James. / A new diagnostic for cancer dynamics : Status and initial tests of the NANIVID. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7207 2009.
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