A new diagnostic for cancer dynamics: Status and initial tests of the NANIVID

Waseem K. Raja, Bojana Gligorijevic, John S. Condeelis, James Castracane

Research output: Contribution to journalConference article

4 Scopus citations

Abstract

The Tumor MicroEnvironment for Metastasis (TMEM) is a critical determinant which will presage the evolution of primary tumors and the resulting metastatic dynamics. Primary tumor cells up and down regulate certain genes which increase motility and cause a disregard for positional information. We report on the development of a new tool for the documentation of cancer cell migration (initial targets: the rat mammary adenocarcinoma cell lines MTLn3 with an over expression of Mena+++). This tool, the NANo IntraVital Device (NANIVID), is a multi-functional nanosystem composed of a chemoattractant source (hydrogel-EGF), capsule (cell trap), counter (transparent, interdigitated electrode arrays for sensing cell arrival), and remote reporter (readout electronics). The device will be retrieved from the tumor site and the cells will be expelled for subsequent assay. The NANIVID will be used in conjunction with the current catheter-based approach in which a needle is loaded with a chemoattractant source and injected into the tumor. A major drawback in the catheter approach is the short cell collection time and lack of real time registering and reporting of cell arrival. This paper will present the current status of the NANIVID prototypes developed in which a transparent implantable device is loaded with chemoattractant source and placed near candidate mammary gland tumors in an established rat model for multiple days or weeks. This series of experiments will allow the comparison of methods and to benchmark the NANIVID for use in research. Initial results of these experiments and NANIVID design modifications will be presented.

Original languageEnglish (US)
Article number72070E
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7207
DOIs
StatePublished - May 5 2009
EventMicrofluidics, BioMEMS, and Medical Microsystems VII - San Jose, CA, United States
Duration: Jan 26 2009Jan 28 2009

Keywords

  • Controlled release
  • ELISA
  • Epidermal growth factor (EGF)
  • Hydrogel
  • Indium doped tin oxide electrodes
  • Poly (ethylene glycol) DiAcrylate (PEGDA)
  • Tumor cells

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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