A new chemotaxis device for cell migration studies

Waseem Khan Raja, Bojana Gligorijevic, Jeff Wyckoff, John S. Condeelis, James Castracane

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

This study presents the design and optimization for in vitro use of a new versatile chemotaxis device called the NANIVID (NANo IntraVital Imaging Device), developed using advanced nano/micro fabrication techniques. The device is fabricated using microphotolithographic techniques and two substrates are bonded together using a thin polymer layer creating a sealed device with one outlet. The main structure of the device consists of two Pyrex substrates: an etched chemoattractant reservoir and a top cover, with a final size of 0.2 × 2 × 3 mm. This reservoir contains a hydrogel blend with EGF which diffuses out through a small (∼9.103 μm2) outlet. This reservoir sustains a steady release of growth factor into the surrounding environment for several hours establishing a consistent concentration gradient from the device. The focus of this study was to design and optimize the new device for cell chemotaxis studies in breast cancer cells in cell culture. Our results show that we have created a flexible, cheap, miniature and autonomous chemotaxis device and demonstrate its usefulness in 2D and 3D cell culture. We also provide preliminary data for use of the device in vivo.

Original languageEnglish (US)
Pages (from-to)696-706
Number of pages11
JournalIntegrative Biology
Volume2
Issue number11-12
DOIs
StatePublished - Nov 2010

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Chemotaxis
Cell culture
Cell Movement
Hermetic devices
Equipment and Supplies
Hydrogel
Microfabrication
Chemotactic Factors
Substrates
Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
Polymers
Cells
Imaging techniques
Cell Culture Techniques
Breast Neoplasms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Raja, W. K., Gligorijevic, B., Wyckoff, J., Condeelis, J. S., & Castracane, J. (2010). A new chemotaxis device for cell migration studies. Integrative Biology, 2(11-12), 696-706. https://doi.org/10.1039/c0ib00044b

A new chemotaxis device for cell migration studies. / Raja, Waseem Khan; Gligorijevic, Bojana; Wyckoff, Jeff; Condeelis, John S.; Castracane, James.

In: Integrative Biology, Vol. 2, No. 11-12, 11.2010, p. 696-706.

Research output: Contribution to journalArticle

Raja, WK, Gligorijevic, B, Wyckoff, J, Condeelis, JS & Castracane, J 2010, 'A new chemotaxis device for cell migration studies', Integrative Biology, vol. 2, no. 11-12, pp. 696-706. https://doi.org/10.1039/c0ib00044b
Raja WK, Gligorijevic B, Wyckoff J, Condeelis JS, Castracane J. A new chemotaxis device for cell migration studies. Integrative Biology. 2010 Nov;2(11-12):696-706. https://doi.org/10.1039/c0ib00044b
Raja, Waseem Khan ; Gligorijevic, Bojana ; Wyckoff, Jeff ; Condeelis, John S. ; Castracane, James. / A new chemotaxis device for cell migration studies. In: Integrative Biology. 2010 ; Vol. 2, No. 11-12. pp. 696-706.
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