A multisubunit particle implicated in membrane fusion

Duncan W. Wilson, Sidney W. Whiteheart, Martin Wiedmann, Michael Brunner, James E. Rothman

Research output: Contribution to journalArticle

198 Citations (Scopus)

Abstract

The N-ethylmaleimide sensitive fusion protein (NSF) is required for fusion of lipid bilayers at many locations within eukaryotic cells. Binding of NSF to Golgi membranes is known to require an integral membrane receptor and one or more members of a family of related soluble NSF attachment proteins (α-, β-, and γ-SNAPs). Here we demonstrate the direct interaction of NSF, SNAPs and an integral membrane component in a detergent solubilized system. We show that NSF only binds to SNAPs in the presence of the integral receptor, resulting in the formation of a multisubunit protein complex with a sedimentation coefficient of 20S. Particle assembly reveals striking differences between members of the SNAP protein family; γ-SNAP associates with the complex via a binding site distinct from that used by α- and β-SNAPs, which are themselves equivalent, alternative subunits of the particle. Once formed, the 20S particle is subsequently able to disassemble in a process coupled to the hydrolysis of ATP. We suggest how cycles of complex assembly and disassembly could help confer specificity to the generalized NSF-dependent fusion apparatus.

Original languageEnglish (US)
Pages (from-to)531-538
Number of pages8
JournalJournal of Cell Biology
Volume117
Issue number3
StatePublished - May 1992
Externally publishedYes

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N-Ethylmaleimide-Sensitive Proteins
Membrane Fusion
Membranes
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Proteins
Lipid Bilayers
Eukaryotic Cells
Detergents
Hydrolysis
Adenosine Triphosphate
Binding Sites

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wilson, D. W., Whiteheart, S. W., Wiedmann, M., Brunner, M., & Rothman, J. E. (1992). A multisubunit particle implicated in membrane fusion. Journal of Cell Biology, 117(3), 531-538.

A multisubunit particle implicated in membrane fusion. / Wilson, Duncan W.; Whiteheart, Sidney W.; Wiedmann, Martin; Brunner, Michael; Rothman, James E.

In: Journal of Cell Biology, Vol. 117, No. 3, 05.1992, p. 531-538.

Research output: Contribution to journalArticle

Wilson, DW, Whiteheart, SW, Wiedmann, M, Brunner, M & Rothman, JE 1992, 'A multisubunit particle implicated in membrane fusion', Journal of Cell Biology, vol. 117, no. 3, pp. 531-538.
Wilson DW, Whiteheart SW, Wiedmann M, Brunner M, Rothman JE. A multisubunit particle implicated in membrane fusion. Journal of Cell Biology. 1992 May;117(3):531-538.
Wilson, Duncan W. ; Whiteheart, Sidney W. ; Wiedmann, Martin ; Brunner, Michael ; Rothman, James E. / A multisubunit particle implicated in membrane fusion. In: Journal of Cell Biology. 1992 ; Vol. 117, No. 3. pp. 531-538.
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