A multicolor podocyte reporter highlights heterogeneous podocyte changes in focal segmental glomerulosclerosis

Jianling Tao, Christina Polumbo, Kimberly Reidy, Mariya Sweetwyne, Katalin Susztak

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In contrast to most glomerular diseases, the injury pattern in focal segmental glomerulosclerosis (FSGS) is highly heterogeneous, even though podocytes are genetically identical and exposed to the same environmental factors. To understand changes in individual podocytes, we generated and analyzed a stochastic multicolor Cre-reporter, encoding four fluorescent proteins. In these animals, podocytes were randomly labeled allowing individual cells and their foot processes to be distinguished. In healthy animals, podocyte size and structure showed little cell to cell variability. In the doxorubicin-induced FSGS model, fluorescent-labeled glomerular podocyte numbers decreased and fluorescent cells could be recovered from the urine. The size of the remaining podocytes showed a high degree of heterogeneity, some cells remained small, while others enlarged. Both enlarged and non-enlarged podocytes showed alterations in their foot process morphology. Thus, by the virtue of a multicolor cre-reporter, individual podocytes could be viewed in real time at a cellular resolution indicating a heterogeneous podocyte injury response during the pathogenesis of FSGS.

Original languageEnglish (US)
Pages (from-to)972-980
Number of pages9
JournalKidney international
Volume85
Issue number4
DOIs
StatePublished - Apr 2014

Keywords

  • Focal segmental glomerulosclerosis
  • Foot process effacement
  • Glomerulosclerosis
  • Multicolor reporter
  • Podocyte

ASJC Scopus subject areas

  • Nephrology

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