A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen in caenorhabditis elegans

Jennifer D. Cohen, Alessandro P. Sparacio, Alexandra C. Belfi, Rachel Forman-Rubinsky, David H. Hall, Hannah M. Maul-Newby, Alison R. Frand, Meera V. Sundaram

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Biological tubes must develop and maintain their proper diameter in order to transport materials efficiently. These tubes are molded and protected in part by apical extracellular matrices (aECMs) that line their lumens. Despite their importance, aECMs are difficult to image in vivo and therefore poorly understood. The C. elegans vulva has been a paradigm for understanding many aspects of organogenesis. Here we describe the vulva luminal matrix, which contains chondroitin proteoglycans, Zona Pellucida (ZP) domain proteins, and other glycoproteins and lipid transporters related to those in mammals. Confocal and transmission electron microscopy revealed, with unprecedented detail, a complex and dynamic aECM. Different matrix factors assemble on the apical surfaces of each vulva cell type, with clear distinctions seen between Ras-dependent (1˚) and Notch-dependent (2˚) cell types. Genetic perturbations suggest that chondroitin and other aECM factors together generate a structured scaffold that both expands and constricts lumen shape.

Original languageEnglish (US)
Pages (from-to)1-77
Number of pages77
JournaleLife
Volume9
DOIs
StatePublished - Sep 2020

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen in caenorhabditis elegans'. Together they form a unique fingerprint.

Cite this