A model system for the study of heteroexchange diffusion: Methotrexate-folate interactions in L1210 leukemia and Ehrlich ascites tumor cells

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Abstract

A unique interaction between the folate analog, methotrexate (4-amino-4-deoxy-10-methylpteroylglutamic acid), and the naturally occurring folates in L1210 leukemia and Ehrlich ascites tumor cells provides a useful model for the study of heteroexchange diffusion. The presence of intracellular binding sites with a high affinity for methotrexate but a low affinity for folic acid and its tetrahydrofolate derivatives permit the measurement of true unidirectional influx rates for methotrexate and assure that the trans-stimulation of methotrexate uptake by the intracellular presence of the other folates is due solely to a primary augmentation of this carrier influx mechanism. Further, since free methotrexate does not appear prior to saturation of the binding sites, the reaction between the folates and carrier at the inner cell membrane is undisturbed by methotrexate released from carrier as the complex enters the cell during heteroexchange, facilitating quantitation of the kinetic alterations which occur for methotrexate influx during trans-stimulation. Trans-stimulation of methotrexate influx was observed in cells preloaded with 5-formyl-, or 5-methyl-tetrahydrofolate, or folic acid. The latter was observed even when intracellular reduction of folic acid to its tetrahydrofolate derivatives was inhibited. However, initial uptake rates for methotrexate were unaltered in cells preloaded with nonlabeled methotrexate. Methotrexate influx conformed to Michaelis-Menten kinetics in L1210 cells preloaded to a single intracellular 5-formyltetrahydrofolate level and when compared to control cells, manifested an increase in the influx vmax without a significant change in the influx Kt. The initial uptake rate for 5-methyltetrahydrofolate was increased in cells preloaded with either 5-formyltetrahydrofolate or folic acid. These studies further support a carrier transport mechanism for methotrexate and the naturally occurring folates in L1210 leukemia cells and extend these findings to the Ehrlich ascites tumor cell as well.

Original languageEnglish (US)
Pages (from-to)624-634
Number of pages11
JournalBBA - Biomembranes
Volume223
Issue number3
DOIs
StatePublished - Jun 1 1971
Externally publishedYes

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Leukemia L1210
Ehrlich Tumor Carcinoma
Folic Acid
Methotrexate
Tumors
Cells
Leucovorin
Binding Sites
Derivatives
Kinetics
Carrier transport
Cell membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

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title = "A model system for the study of heteroexchange diffusion: Methotrexate-folate interactions in L1210 leukemia and Ehrlich ascites tumor cells",
abstract = "A unique interaction between the folate analog, methotrexate (4-amino-4-deoxy-10-methylpteroylglutamic acid), and the naturally occurring folates in L1210 leukemia and Ehrlich ascites tumor cells provides a useful model for the study of heteroexchange diffusion. The presence of intracellular binding sites with a high affinity for methotrexate but a low affinity for folic acid and its tetrahydrofolate derivatives permit the measurement of true unidirectional influx rates for methotrexate and assure that the trans-stimulation of methotrexate uptake by the intracellular presence of the other folates is due solely to a primary augmentation of this carrier influx mechanism. Further, since free methotrexate does not appear prior to saturation of the binding sites, the reaction between the folates and carrier at the inner cell membrane is undisturbed by methotrexate released from carrier as the complex enters the cell during heteroexchange, facilitating quantitation of the kinetic alterations which occur for methotrexate influx during trans-stimulation. Trans-stimulation of methotrexate influx was observed in cells preloaded with 5-formyl-, or 5-methyl-tetrahydrofolate, or folic acid. The latter was observed even when intracellular reduction of folic acid to its tetrahydrofolate derivatives was inhibited. However, initial uptake rates for methotrexate were unaltered in cells preloaded with nonlabeled methotrexate. Methotrexate influx conformed to Michaelis-Menten kinetics in L1210 cells preloaded to a single intracellular 5-formyltetrahydrofolate level and when compared to control cells, manifested an increase in the influx vmax without a significant change in the influx Kt. The initial uptake rate for 5-methyltetrahydrofolate was increased in cells preloaded with either 5-formyltetrahydrofolate or folic acid. These studies further support a carrier transport mechanism for methotrexate and the naturally occurring folates in L1210 leukemia cells and extend these findings to the Ehrlich ascites tumor cell as well.",
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N2 - A unique interaction between the folate analog, methotrexate (4-amino-4-deoxy-10-methylpteroylglutamic acid), and the naturally occurring folates in L1210 leukemia and Ehrlich ascites tumor cells provides a useful model for the study of heteroexchange diffusion. The presence of intracellular binding sites with a high affinity for methotrexate but a low affinity for folic acid and its tetrahydrofolate derivatives permit the measurement of true unidirectional influx rates for methotrexate and assure that the trans-stimulation of methotrexate uptake by the intracellular presence of the other folates is due solely to a primary augmentation of this carrier influx mechanism. Further, since free methotrexate does not appear prior to saturation of the binding sites, the reaction between the folates and carrier at the inner cell membrane is undisturbed by methotrexate released from carrier as the complex enters the cell during heteroexchange, facilitating quantitation of the kinetic alterations which occur for methotrexate influx during trans-stimulation. Trans-stimulation of methotrexate influx was observed in cells preloaded with 5-formyl-, or 5-methyl-tetrahydrofolate, or folic acid. The latter was observed even when intracellular reduction of folic acid to its tetrahydrofolate derivatives was inhibited. However, initial uptake rates for methotrexate were unaltered in cells preloaded with nonlabeled methotrexate. Methotrexate influx conformed to Michaelis-Menten kinetics in L1210 cells preloaded to a single intracellular 5-formyltetrahydrofolate level and when compared to control cells, manifested an increase in the influx vmax without a significant change in the influx Kt. The initial uptake rate for 5-methyltetrahydrofolate was increased in cells preloaded with either 5-formyltetrahydrofolate or folic acid. These studies further support a carrier transport mechanism for methotrexate and the naturally occurring folates in L1210 leukemia cells and extend these findings to the Ehrlich ascites tumor cell as well.

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