A model for transcription termination by RNA polymerase I

Walter H. Lang; Bernice E. Morrow; Qida Ju; Jonathan R. Warner; Ronald H. Reeder

doi:10.1016/0092-8674(94)90261-5

A model for transcription termination by RNA polymerase I

Walter H. Lang, Bernice E. Morrow, Qida Ju, Jonathan R. Warner, Ronald H. Reeder

Genetics

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

Abstract

The transcription termination site for yeast RNA polymerase I requires not only an 11 bp binding site for Reb1p, but also about 46 bp of 5′ flanking sequence. We propose that Reb1p bound to its site is part of a pause element, while the 5′ flanking sequence contains a release element. Pausing requires little other than the DNA-binding domain of Reb1p and is not specific for polymerase I. The release element, however, can be polymerase specific. We propose a general model for eukaryotic transcription terminators in which termination occurs when a relatively nonspecific signal induces polymerase to pause in the context of a release element.

Original language	English (US)
Pages (from-to)	527-534
Number of pages	8
Journal	Cell
Volume	79
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(94)90261-5
State	Published - Nov 4 1994

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(94)90261-5

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title = "A model for transcription termination by RNA polymerase I",

abstract = "The transcription termination site for yeast RNA polymerase I requires not only an 11 bp binding site for Reb1p, but also about 46 bp of 5′ flanking sequence. We propose that Reb1p bound to its site is part of a pause element, while the 5′ flanking sequence contains a release element. Pausing requires little other than the DNA-binding domain of Reb1p and is not specific for polymerase I. The release element, however, can be polymerase specific. We propose a general model for eukaryotic transcription terminators in which termination occurs when a relatively nonspecific signal induces polymerase to pause in the context of a release element.",

author = "Lang, {Walter H.} and Morrow, {Bernice E.} and Qida Ju and Warner, {Jonathan R.} and Reeder, {Ronald H.}",

note = "Funding Information: Judith Roan provided excellent technical assistance. This work was partially supported by National Institutes of Health grants GM41 792 to R. H. R. and GM25532 to J. R. W.",

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T1 - A model for transcription termination by RNA polymerase I

AU - Lang, Walter H.

AU - Morrow, Bernice E.

AU - Ju, Qida

AU - Warner, Jonathan R.

AU - Reeder, Ronald H.

N1 - Funding Information: Judith Roan provided excellent technical assistance. This work was partially supported by National Institutes of Health grants GM41 792 to R. H. R. and GM25532 to J. R. W.

PY - 1994/11/4

Y1 - 1994/11/4

N2 - The transcription termination site for yeast RNA polymerase I requires not only an 11 bp binding site for Reb1p, but also about 46 bp of 5′ flanking sequence. We propose that Reb1p bound to its site is part of a pause element, while the 5′ flanking sequence contains a release element. Pausing requires little other than the DNA-binding domain of Reb1p and is not specific for polymerase I. The release element, however, can be polymerase specific. We propose a general model for eukaryotic transcription terminators in which termination occurs when a relatively nonspecific signal induces polymerase to pause in the context of a release element.

AB - The transcription termination site for yeast RNA polymerase I requires not only an 11 bp binding site for Reb1p, but also about 46 bp of 5′ flanking sequence. We propose that Reb1p bound to its site is part of a pause element, while the 5′ flanking sequence contains a release element. Pausing requires little other than the DNA-binding domain of Reb1p and is not specific for polymerase I. The release element, however, can be polymerase specific. We propose a general model for eukaryotic transcription terminators in which termination occurs when a relatively nonspecific signal induces polymerase to pause in the context of a release element.

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A model for transcription termination by RNA polymerase I

Abstract

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