A mobile-phone based high-resolution microendoscope to image cervical precancer

Benjamin D. Grant, Timothy Quang, Júlio César Possati-Resende, Cristovam Scapulatempo-Neto, Graziela de Macedo Matsushita, Edmundo Carvalho Mauad, Mark H. Stoler, Philip E. Castle, José Humberto Tavares Guerreiro Fregnani, Kathleen M. Schmeler, Rebecca Richards-Kortum

Research output: Contribution to journalArticle

Abstract

Nearly 90% of cervical cancer cases and deaths occur in low- and middle-income countries that lack comprehensive national HPV immunization and cervical cancer screening programs. In these settings, it is difficult to implement screening programs due to a lack of infrastructure and shortage of trained personnel. Screening programs based on visual inspection with acetic acid (VIA) have been successfully implemented in some low-resource settings. However, VIA has poor specificity and up to 90% of patients receiving treatment based on a positive VIA exam are over-treated. A number of studies have suggested that high-resolution cervical imaging to visualize nuclear morphology in vivo can improve specificity by better distinguishing precancerous and benign lesions. To enable high-resolution imaging in low-resource settings, we developed a portable, low-cost, high-resolution microendoscope that uses a mobile phone to detect and display images of cervical epithelium in vivo with subcellular resolution. The device was fabricated for less than $2,000 using commercially available optical components including filters, an LED and triplet lenses assembled in a 3D-printed opto-mechanical mount. We show that the mobile high-resolution microendoscope achieves similar resolution and signal-to-background ratio as previously reported high-resolution microendoscope systems using traditional cameras and computers to detect and display images. Finally, we demonstrate the ability of the mobile high-resolution microendoscope to image normal and precancerous squamous epithelium of the cervix in vivo in a gynecological referral clinic in Barretos, Brazil.

Original languageEnglish (US)
Article numbere0211045
JournalPLoS One
Volume14
Issue number2
DOIs
StatePublished - Feb 1 2019

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Cell Phones
Mobile phones
Acetic Acid
acetic acid
Screening
uterine cervical neoplasms
Inspection
screening
Uterine Cervical Neoplasms
epithelium
Epithelium
image analysis
Immunization
Imaging techniques
Optical Devices
cervix
Lens
Early Detection of Cancer
infrastructure
Cervix Uteri

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Grant, B. D., Quang, T., Possati-Resende, J. C., Scapulatempo-Neto, C., de Macedo Matsushita, G., Mauad, E. C., ... Richards-Kortum, R. (2019). A mobile-phone based high-resolution microendoscope to image cervical precancer. PLoS One, 14(2), [e0211045]. https://doi.org/10.1371/journal.pone.0211045

A mobile-phone based high-resolution microendoscope to image cervical precancer. / Grant, Benjamin D.; Quang, Timothy; Possati-Resende, Júlio César; Scapulatempo-Neto, Cristovam; de Macedo Matsushita, Graziela; Mauad, Edmundo Carvalho; Stoler, Mark H.; Castle, Philip E.; Guerreiro Fregnani, José Humberto Tavares; Schmeler, Kathleen M.; Richards-Kortum, Rebecca.

In: PLoS One, Vol. 14, No. 2, e0211045, 01.02.2019.

Research output: Contribution to journalArticle

Grant, BD, Quang, T, Possati-Resende, JC, Scapulatempo-Neto, C, de Macedo Matsushita, G, Mauad, EC, Stoler, MH, Castle, PE, Guerreiro Fregnani, JHT, Schmeler, KM & Richards-Kortum, R 2019, 'A mobile-phone based high-resolution microendoscope to image cervical precancer', PLoS One, vol. 14, no. 2, e0211045. https://doi.org/10.1371/journal.pone.0211045
Grant BD, Quang T, Possati-Resende JC, Scapulatempo-Neto C, de Macedo Matsushita G, Mauad EC et al. A mobile-phone based high-resolution microendoscope to image cervical precancer. PLoS One. 2019 Feb 1;14(2). e0211045. https://doi.org/10.1371/journal.pone.0211045
Grant, Benjamin D. ; Quang, Timothy ; Possati-Resende, Júlio César ; Scapulatempo-Neto, Cristovam ; de Macedo Matsushita, Graziela ; Mauad, Edmundo Carvalho ; Stoler, Mark H. ; Castle, Philip E. ; Guerreiro Fregnani, José Humberto Tavares ; Schmeler, Kathleen M. ; Richards-Kortum, Rebecca. / A mobile-phone based high-resolution microendoscope to image cervical precancer. In: PLoS One. 2019 ; Vol. 14, No. 2.
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