A microassay for colony-stimulating factor based on thymidine incorporation

M. B. Prystowsky, M. F. Naujokas, J. N. Ihle, E. Goldwasser, F. W. Fitch

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12 Scopus citations

Abstract

A variety of growth factors and lectins were tested; only colony-stimulating factors CSF-1, Interleukin 3, and a T-lymphocyte GM CSF induced colony formation in semisolid medium and stimulated thymidine incorporation in liquid culture. All other growth factors and lectins were inactive in both assays. Factor-stimulated thymidine incorporation was detectable 24 hours after stimulation and reached maximal levels 4-6 days after stimulation. A convenient microassay for measuring CSF activity has been developed, enabling a large number of samples to be screened qualitatively in 2 days and permitting CSF activity to be measured quantitatively in 4-5 days. This microassay can supplement in the clonal-cell assay method and be especially useful as an initial screening assay for CSF activity.

Original languageEnglish (US)
Pages (from-to)149-156
Number of pages8
JournalAmerican Journal of Pathology
Volume114
Issue number1
StatePublished - Mar 22 1984
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Prystowsky, M. B., Naujokas, M. F., Ihle, J. N., Goldwasser, E., & Fitch, F. W. (1984). A microassay for colony-stimulating factor based on thymidine incorporation. American Journal of Pathology, 114(1), 149-156.