@article{2f302babae394096acd469cced698f16,
title = "A method for the morphological identification of contractile filaments in single cells",
abstract = "A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.",
author = "Moore, {P. L.} and Condeelis, {J. S.} and Taylor, {D. L.} and Allen, {R. D.}",
note = "Funding Information: The authors thank Dr Norma Lang for cultures of Nauiculu and Dr DennisR aveling for the use of his stop-action movie projector. This study was supported by NSF grant no. GB 30751 and Cancer Research Funds from the University of California 1. Ambrose, E J, Exptl cell res, suppl. 8 (1961) 54. 2. Halfen, L N & Castenholz, R W, Nature 225 (1970) 1163. 3. - J phycol 7 (1971) 133. 4. Wessells. N K. Swooner. B S. Ash. J F. Bradlev. M 0, Luduena, M A, Taylor; E L,{\textquoteleft}Wrenn, J T $ Yamada, K M, Science 171 (1970) 135. 5. Spooner, B S, Yamada, K M & Wessells, N K, J cell biol 49 (1971) 595. 6. Yamada, K M, Spooner, B S & Wessells, N K, J cell biol 49 (1971) 614. 7. Williamson, d E, J cell sci 10 (1972) 811. 8. Bradlev. M 0. J cell sci 12 (1973) 327. 9. Arms&&g, P B & Parenti, D; J cell biol 55 I1 972) 542. 10. Schroeder, T E, Biol bull 137 (1969) 413. 11. Colby, R H & Lanners, N, Abstr 1 I th ann meet Am sot cell biol, New Orleans, p. 60 (1971). 12. Schroeder. T E. Z Zellforsch 109 (1970) 431. 13. Estensen, {\textquoteleft}R D; Rosenberg, M &-Sheridan, J D, Science 173 (1971) 356. 14. Sanger, J W & Holtzer, H, Proc natl acad sci US 69 (1972) 253. 15. Cloney, k A, Z Zellforsch 132 (1972) 167. 16. Manasek. F J. Bumside. B & Stroman. J. Proc natl acad{\textquoteright}sci US 69 (1972) 308. 17. Spooner, B S & Wessells, N K, Dev bio127 (1972) 38. 18. Goldman, R D & Follett, E A C, Exptl cell res 57 (1969) 263. 19. Wagner, G, Haupt, W & Loux, A, Science 176 (1972) 808. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1973",
month = aug,
doi = "10.1016/0014-4827(73)90332-7",
language = "English (US)",
volume = "80",
pages = "493--495",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",
}