Abstract
A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 493-495 |
| Number of pages | 3 |
| Journal | Experimental Cell Research |
| Volume | 80 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1973 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
A method for the morphological identification of contractile filaments in single cells. / Moore, P. L.; Condeelis, John S.; Taylor, D. L.; Allen, R. D.
In: Experimental Cell Research, Vol. 80, No. 2, 1973, p. 493-495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A method for the morphological identification of contractile filaments in single cells
AU - Moore, P. L.
AU - Condeelis, John S.
AU - Taylor, D. L.
AU - Allen, R. D.
PY - 1973
Y1 - 1973
N2 - A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.
AB - A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.
UR - http://www.scopus.com/inward/record.url?scp=0015813319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015813319&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(73)90332-7
DO - 10.1016/0014-4827(73)90332-7
M3 - Article
C2 - 4126816
AN - SCOPUS:0015813319
VL - 80
SP - 493
EP - 495
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -