A method for the morphological identification of contractile filaments in single cells

P. L. Moore, J. S. Condeelis, D. L. Taylor, R. D. Allen

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.

Original languageEnglish (US)
Pages (from-to)493-495
Number of pages3
JournalExperimental Cell Research
Volume80
Issue number2
DOIs
StatePublished - Aug 1973
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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