A method for the morphological identification of contractile filaments in single cells

P. L. Moore; J. S. Condeelis; D. L. Taylor; R. D. Allen

doi:10.1016/0014-4827(73)90332-7

A method for the morphological identification of contractile filaments in single cells

P. L. Moore, J. S. Condeelis, D. L. Taylor, R. D. Allen

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12 Scopus citations

Abstract

A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.

Original language	English (US)
Pages (from-to)	493-495
Number of pages	3
Journal	Experimental Cell Research
Volume	80
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(73)90332-7
Publication status	Published - Aug 1973
Externally published	Yes

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ASJC Scopus subject areas

Cell Biology

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Moore, P. L., Condeelis, J. S., Taylor, D. L., & Allen, R. D. (1973). A method for the morphological identification of contractile filaments in single cells. Experimental Cell Research, 80(2), 493-495. https://doi.org/10.1016/0014-4827(73)90332-7

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AB - A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.

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10.1016/0014-4827(73)90332-7