Abstract
A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 493-495 |
| Number of pages | 3 |
| Journal | Experimental Cell Research |
| Volume | 80 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1973 |
| Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology
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Cite this
Moore, P. L., Condeelis, J. S., Taylor, D. L., & Allen, R. D. (1973). A method for the morphological identification of contractile filaments in single cells. Experimental Cell Research, 80(2), 493-495. https://doi.org/10.1016/0014-4827(73)90332-7