A method for the morphological identification of contractile filaments in single cells

P. L. Moore; J. S. Condeelis; D. L. Taylor; R. D. Allen

doi:10.1016/0014-4827(73)90332-7

A method for the morphological identification of contractile filaments in single cells

P. L. Moore, J. S. Condeelis, D. L. Taylor, R. D. Allen

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.

Original language	English (US)
Pages (from-to)	493-495
Number of pages	3
Journal	Experimental Cell Research
Volume	80
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(73)90332-7
State	Published - Aug 1973
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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title = "A method for the morphological identification of contractile filaments in single cells",

abstract = "A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.",

author = "Moore, {P. L.} and Condeelis, {J. S.} and Taylor, {D. L.} and Allen, {R. D.}",

year = "1973",

month = aug,

doi = "10.1016/0014-4827(73)90332-7",

language = "English (US)",

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pages = "493--495",

journal = "Experimental Cell Research",

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T1 - A method for the morphological identification of contractile filaments in single cells

AU - Moore, P. L.

AU - Condeelis, J. S.

AU - Taylor, D. L.

AU - Allen, R. D.

PY - 1973/8

Y1 - 1973/8

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AB - A simple negative staining procedure has been developed for the demonstration of actin filaments and myosin aggregates in single giant amoeba (Chaos carolinensis) that is applicable to other single cells. Cytoplasm is first isolated in physiological solutions in which contractility and state of association of contractile proteins can be controlled. Cytoplasm isolated in low calcium, low ATP concentration solutions contains actin associated with myosin aggregates sometimes forming light-microscopically visible fibrils. When exogenous ATP is added to these preparations, actin filaments and myosin aggregates are seen separately.

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JF - Experimental Cell Research

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