A method for rapid regulation of protein expression in Trypanosoma cruzi

Yan Fen Ma; Louis M. Weiss; Huan Huang

doi:10.1016/j.ijpara.2011.11.002

A method for rapid regulation of protein expression in Trypanosoma cruzi

Yan Fen Ma, Louis M. Weiss, Huan Huang

Pathology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.

Original language	English (US)
Pages (from-to)	33-37
Number of pages	5
Journal	International Journal for Parasitology
Volume	42
Issue number	1
DOIs	https://doi.org/10.1016/j.ijpara.2011.11.002
State	Published - Jan 2012

Keywords

Chagas disease
Destabilisation domain
Genetic manipulation
PTREX vector
Protein expression
Trypanosoma cruzi

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijpara.2011.11.002

Cite this

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title = "A method for rapid regulation of protein expression in Trypanosoma cruzi",

abstract = "Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.",

keywords = "Chagas disease, Destabilisation domain, Genetic manipulation, PTREX vector, Protein expression, Trypanosoma cruzi",

author = "Ma, {Yan Fen} and Weiss, {Louis M.} and Huan Huang",

note = "Funding Information: This work was supported by National Institutes of Health, USA , Grants AI 058893 (HH) and AI076248 .",

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AU - Ma, Yan Fen

AU - Weiss, Louis M.

AU - Huang, Huan

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PY - 2012/1

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N2 - Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.

AB - Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.

KW - Chagas disease

KW - Destabilisation domain

KW - Genetic manipulation

KW - PTREX vector

KW - Protein expression

KW - Trypanosoma cruzi

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A method for rapid regulation of protein expression in Trypanosoma cruzi

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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