A method for rapid regulation of protein expression in Trypanosoma cruzi

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5 Citations (Scopus)

Abstract

Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalInternational Journal for Parasitology
Volume42
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Trypanosoma cruzi
Proteins
Life Cycle Stages
Ligands
Genes

Keywords

  • Chagas disease
  • Destabilisation domain
  • Genetic manipulation
  • Protein expression
  • PTREX vector
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

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abstract = "Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.",
keywords = "Chagas disease, Destabilisation domain, Genetic manipulation, Protein expression, PTREX vector, Trypanosoma cruzi",
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AB - Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.

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KW - Protein expression

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