A metabolic prosurvival role for PML in breast cancer

Arkaitz Carracedo, Dror Weiss, Amy K. Leliaert, Manoj Bhasin, Vincent C.J. De Boer, Gaelle Laurent, Andrew C. Adams, Maria Sundvall, Su Jung Song, Keisuke Ito, Lydia S. Finley, Ainara Egia, Towia Libermann, Zachary Gerhart-Hines, Pere Puigserver, Marcia C. Haigis, Elefteria Maratos-Flier, Andrea L. Richardson, Zachary T. Schafer, Pier P. Pandolfi

Research output: Contribution to journalArticle

143 Scopus citations

Abstract

Cancer cells exhibit an aberrant metabolism that facilitates more efficient production of biomass and hence tumor growth and progression. However, the genetic cues modulating this metabolic switch remain largely undetermined. We identified a metabolic function for the promyelocytic leukemia (PML) gene, uncovering an unexpected role for this bona fide tumor suppressor in breast cancer cell survival. We found that PML acted as both a negative regulator of PPARγ coactivator 1A (PGC1A) acetylation and a potent activator of PPAR signaling and fatty acid oxidation. We further showed that PML promoted ATP production and inhibited anoikis. Importantly, PML expression allowed luminal filling in 3D basement membrane breast culture models, an effect that was reverted by the pharmacological inhibition of fatty acid oxidation. Additionally, immunohistochemical analysis of breast cancer biopsies revealed that PML was overexpressed in a subset of breast cancers and enriched in triple-negative cases. Indeed, PML expression in breast cancer correlated strikingly with reduced time to recurrence, a gene signature of poor prognosis, and activated PPAR signaling. These findings have important therapeutic implications, as PML and its key role in fatty acid oxidation metabolism are amenable to pharmacological suppression, a potential future mode of cancer prevention and treatment.

Original languageEnglish (US)
Pages (from-to)3088-3100
Number of pages13
JournalJournal of Clinical Investigation
Volume122
Issue number9
DOIs
StatePublished - Sep 4 2012

ASJC Scopus subject areas

  • Medicine(all)

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    Carracedo, A., Weiss, D., Leliaert, A. K., Bhasin, M., De Boer, V. C. J., Laurent, G., Adams, A. C., Sundvall, M., Song, S. J., Ito, K., Finley, L. S., Egia, A., Libermann, T., Gerhart-Hines, Z., Puigserver, P., Haigis, M. C., Maratos-Flier, E., Richardson, A. L., Schafer, Z. T., & Pandolfi, P. P. (2012). A metabolic prosurvival role for PML in breast cancer. Journal of Clinical Investigation, 122(9), 3088-3100. https://doi.org/10.1172/JCI62129