A medium-chain fatty acid as an alternative energy source in mouse preimplantation development

Mitsutoshi Yamada, Kazumi Takanashi, Toshio Hamatani, Akiyoshi Hirayama, Hidenori Akutsu, Tomoko Fukunaga, Seiji Ogawa, Kana Sugawara, Kosaku Shinoda, Tomoyoshi Soga, Akihiro Umezawa, Naoaki Kuji, Yasunori Yoshimura, Masaru Tomita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To further optimize the culturing of preimplantation embryos, we undertook metabolomic analysis of relevant culture media using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). We detected 28 metabolites: 23 embryo-excreted metabolites including 16 amino acids and 5 media-derived metabolites (e.g., octanoate, a medium-chain fatty acid (MCFA)). Due to the lack of information on MCFAs in mammalian preimplantation development, this study examined octanoate as a potential alternative energy source for preimplantation embryo cultures. No embryos survived in culture media lacking FAs, pyruvate, and glucose, but supplementation of octanoate rescued the embryonic development. Immunoblotting showed significant expression of acyl-CoA dehydrogenase and hydroxyacyl-CoA dehydrogenase, important enzymes for β-oxidation of MCFAs, in preimplantation embryo. Furthermore, CE-TOFMS traced [1-13 C 8[ octanoate added to the culture media into intermediate metabolites of the TCA cycle via β-oxidation in mitochondria. These results are the first demonstration that octanoate could provide an efficient alternative energy source throughout preimplantation development.

Original languageEnglish (US)
Article number930
JournalScientific Reports
Volume2
DOIs
StatePublished - Dec 14 2012
Externally publishedYes

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Fatty Acids
Blastocyst
Culture Media
Capillary Electrophoresis
Mass Spectrometry
Embryonic Structures
Acyl-CoA Dehydrogenase
Metabolomics
Coenzyme A
Pyruvic Acid
Immunoblotting
Embryonic Development
Mitochondria
Oxidoreductases
octanoic acid
Amino Acids
Glucose
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Yamada, M., Takanashi, K., Hamatani, T., Hirayama, A., Akutsu, H., Fukunaga, T., ... Tomita, M. (2012). A medium-chain fatty acid as an alternative energy source in mouse preimplantation development. Scientific Reports, 2, [930]. https://doi.org/10.1038/srep00930

A medium-chain fatty acid as an alternative energy source in mouse preimplantation development. / Yamada, Mitsutoshi; Takanashi, Kazumi; Hamatani, Toshio; Hirayama, Akiyoshi; Akutsu, Hidenori; Fukunaga, Tomoko; Ogawa, Seiji; Sugawara, Kana; Shinoda, Kosaku; Soga, Tomoyoshi; Umezawa, Akihiro; Kuji, Naoaki; Yoshimura, Yasunori; Tomita, Masaru.

In: Scientific Reports, Vol. 2, 930, 14.12.2012.

Research output: Contribution to journalArticle

Yamada, M, Takanashi, K, Hamatani, T, Hirayama, A, Akutsu, H, Fukunaga, T, Ogawa, S, Sugawara, K, Shinoda, K, Soga, T, Umezawa, A, Kuji, N, Yoshimura, Y & Tomita, M 2012, 'A medium-chain fatty acid as an alternative energy source in mouse preimplantation development', Scientific Reports, vol. 2, 930. https://doi.org/10.1038/srep00930
Yamada M, Takanashi K, Hamatani T, Hirayama A, Akutsu H, Fukunaga T et al. A medium-chain fatty acid as an alternative energy source in mouse preimplantation development. Scientific Reports. 2012 Dec 14;2. 930. https://doi.org/10.1038/srep00930
Yamada, Mitsutoshi ; Takanashi, Kazumi ; Hamatani, Toshio ; Hirayama, Akiyoshi ; Akutsu, Hidenori ; Fukunaga, Tomoko ; Ogawa, Seiji ; Sugawara, Kana ; Shinoda, Kosaku ; Soga, Tomoyoshi ; Umezawa, Akihiro ; Kuji, Naoaki ; Yoshimura, Yasunori ; Tomita, Masaru. / A medium-chain fatty acid as an alternative energy source in mouse preimplantation development. In: Scientific Reports. 2012 ; Vol. 2.
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