A magnetic resonance imaging study of intestinal dilation in Trypanosoma cruzi-infected mice deficient in nitric oxide synthase

Lars Ny, Hua Li, Shankar Mukherjee, Katarina Persson, Bo Holmqvist, Dazhi Zhao, Vitaliy Shtutin, Huan Huang, Louis M. Weiss, Fabiana S. Machado, Stephen M. Factor, John Chan, Herbert B. Tanowitz, Linda A. Jelicks

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Infection with Trypanosoma cruzi causes megasyndromes of the gastrointestinal (GI) tract. We used magnetic resonance imaging (MRI) to monitor alterations in the GI tract of T. cruzi-infected mice, and to assess the role of nitric oxide (NO) in the development of intestinal dilation. Brazil strain-infected C57BL/6 wild-type (WT) mice exhibited dilatation of the intestines by 30 days post-infection. Average intestine lumen diameter increased by 72%. Levels of intestinal NO synthase (NOS) isoforms, NOS2 and NOS3, were elevated in infected WT mice. Inflammation and ganglionitis were observed in all infected mice. Intestinal dilation was observed in infected WT, NOS1, NOS2, and NOS3 null mice. This study demonstrates that MRI is a useful tool to monitor intestinal dilation in living mice and that these alterations may begin during acute infection. Furthermore, our data strongly suggests that NO may not be the sole contributor to intestinal dysfunction resulting from this infection.

Original languageEnglish (US)
Pages (from-to)760-767
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume79
Issue number5
StatePublished - Nov 2008

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Trypanosoma cruzi
Nitric Oxide Synthase
Dilatation
Magnetic Resonance Imaging
Infection
Intestines
Gastrointestinal Tract
Nitric Oxide
Brazil
Protein Isoforms
Inflammation

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

A magnetic resonance imaging study of intestinal dilation in Trypanosoma cruzi-infected mice deficient in nitric oxide synthase. / Ny, Lars; Li, Hua; Mukherjee, Shankar; Persson, Katarina; Holmqvist, Bo; Zhao, Dazhi; Shtutin, Vitaliy; Huang, Huan; Weiss, Louis M.; Machado, Fabiana S.; Factor, Stephen M.; Chan, John; Tanowitz, Herbert B.; Jelicks, Linda A.

In: American Journal of Tropical Medicine and Hygiene, Vol. 79, No. 5, 11.2008, p. 760-767.

Research output: Contribution to journalArticle

Ny, L, Li, H, Mukherjee, S, Persson, K, Holmqvist, B, Zhao, D, Shtutin, V, Huang, H, Weiss, LM, Machado, FS, Factor, SM, Chan, J, Tanowitz, HB & Jelicks, LA 2008, 'A magnetic resonance imaging study of intestinal dilation in Trypanosoma cruzi-infected mice deficient in nitric oxide synthase', American Journal of Tropical Medicine and Hygiene, vol. 79, no. 5, pp. 760-767.
Ny, Lars ; Li, Hua ; Mukherjee, Shankar ; Persson, Katarina ; Holmqvist, Bo ; Zhao, Dazhi ; Shtutin, Vitaliy ; Huang, Huan ; Weiss, Louis M. ; Machado, Fabiana S. ; Factor, Stephen M. ; Chan, John ; Tanowitz, Herbert B. ; Jelicks, Linda A. / A magnetic resonance imaging study of intestinal dilation in Trypanosoma cruzi-infected mice deficient in nitric oxide synthase. In: American Journal of Tropical Medicine and Hygiene. 2008 ; Vol. 79, No. 5. pp. 760-767.
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