A large gene network in immature Erythroid cells is controlled by the myeloid and B cell transcriptional regulator PU.1

Sandeep N. Wontakal, Xingyi Guo, Britta Will, Minyi Shi, Debasish Raha, Milind C. Mahajan, Sherman Weissman, Michael Snyder, Ulrich G. Steidl, Deyou Zheng, Arthur I. Skoultchi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

PU.1 is a hematopoietic transcription factor that is required for the development of myeloid and B cells. PU.1 is also expressed in erythroid progenitors, where it blocks erythroid differentiation by binding to and inhibiting the main erythroid promoting factor, GATA-1. However, other mechanisms by which PU.1 affects the fate of erythroid progenitors have not been thoroughly explored. Here, we used ChIP-Seq analysis for PU.1 and gene expression profiling in erythroid cells to show that PU.1 regulates an extensive network of genes that constitute major pathways for controlling growth and survival of immature erythroid cells. By analyzing fetal liver erythroid progenitors from mice with low PU.1 expression, we also show that the earliest erythroid committed cells are dramatically reduced in vivo. Furthermore, we find that PU.1 also regulates many of the same genes and pathways in other blood cells, leading us to propose that PU.1 is a multifaceted factor with overlapping, as well as distinct, functions in several hematopoietic lineages.

Original languageEnglish (US)
Article numbere1001392
JournalPLoS Genetics
Volume7
Issue number6
DOIs
StatePublished - Jun 2011

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Erythroid Cells
Gene Regulatory Networks
Myeloid Cells
B-lymphocytes
B-Lymphocytes
transcription factors
immatures
gene
GATA Transcription Factors
gene expression
blood
Gene Expression Profiling
cells
major genes
blood cells
Blood Cells
Transcription Factors
liver
Liver
mice

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

A large gene network in immature Erythroid cells is controlled by the myeloid and B cell transcriptional regulator PU.1. / Wontakal, Sandeep N.; Guo, Xingyi; Will, Britta; Shi, Minyi; Raha, Debasish; Mahajan, Milind C.; Weissman, Sherman; Snyder, Michael; Steidl, Ulrich G.; Zheng, Deyou; Skoultchi, Arthur I.

In: PLoS Genetics, Vol. 7, No. 6, e1001392, 06.2011.

Research output: Contribution to journalArticle

Wontakal, Sandeep N. ; Guo, Xingyi ; Will, Britta ; Shi, Minyi ; Raha, Debasish ; Mahajan, Milind C. ; Weissman, Sherman ; Snyder, Michael ; Steidl, Ulrich G. ; Zheng, Deyou ; Skoultchi, Arthur I. / A large gene network in immature Erythroid cells is controlled by the myeloid and B cell transcriptional regulator PU.1. In: PLoS Genetics. 2011 ; Vol. 7, No. 6.
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AU - Raha, Debasish

AU - Mahajan, Milind C.

AU - Weissman, Sherman

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