Abstract
The construction of a 200 liter bacterial growth chamber for a total cost of less than $400 is described. The chamber was designed for growth of aerobic organisms and therefore provides high rates of aeration and mixing. The growth of Escherichia coli K12 in the chamber was similar to growth in small flasks on a rotary shaker. Azotobacter vinelandii OP growth is slower in the growth chamber than in small, shaken flasks. Under the growth conditions described, kilogram quantities of bacterial cells are obtained from a single culture.
Original language | English (US) |
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Pages (from-to) | 377-382 |
Number of pages | 6 |
Journal | Analytical Biochemistry |
Volume | 57 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1974 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology