A large, efficient and inexpensive bacterial growth chamber

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The construction of a 200 liter bacterial growth chamber for a total cost of less than $400 is described. The chamber was designed for growth of aerobic organisms and therefore provides high rates of aeration and mixing. The growth of Escherichia coli K12 in the chamber was similar to growth in small flasks on a rotary shaker. Azotobacter vinelandii OP growth is slower in the growth chamber than in small, shaken flasks. Under the growth conditions described, kilogram quantities of bacterial cells are obtained from a single culture.

Original languageEnglish (US)
Pages (from-to)377-382
Number of pages6
JournalAnalytical Biochemistry
Volume57
Issue number2
DOIs
StatePublished - Feb 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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