A Kazal-type serine protease inhibitor from the defense gland secretion of the subterranean termite Coptotermes formosanus Shiraki

Horia Negulescu, Youzhong Guo, Thomas P. Garner, Octavia Y. Goodwin, Gregg Henderson, Roger A. Laine, Megan A. Macnaughtan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Coptotermes formosanus is an imported, subterranean termite species with the largest economic impact in the United States. The frontal glands of the soldier caste termites comprising one third of the body mass, contain a secretion expelled through a foramen in defense. The small molecule composition of the frontal gland secretion is well-characterized, but the proteins remain to be identified. Herein is reported the structure and function of one of several proteins found in the termite defense gland secretion. TFP4 is a 6.9 kDa, non-classical group 1 Kazal-type serine protease inhibitor with activity towards chymotrypsin and elastase, but not trypsin. The 3-dimensional solution structure of TFP4 was solved with nuclear magnetic resonance spectroscopy, and represents the first structure from the taxonomic family, Rhinotermitidae. Based on the structure of TFP4, the protease inhibitor active loop (Cys8 to Cys16) was identified.

Original languageEnglish (US)
Article number0125376
JournalPLoS One
Volume10
Issue number5
DOIs
StatePublished - May 15 2015

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Isoptera
Coptotermes formosanus
subterranean termites
Serine Proteinase Inhibitors
serine proteinases
proteinase inhibitors
secretion
Pancreatic Elastase
Chymotrypsin
Protease Inhibitors
Trypsin
Nuclear magnetic resonance spectroscopy
elastase
Proteins
Rhinotermitidae
Military Personnel
chymotrypsin
economic impact
Social Class
trypsin

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Negulescu, H., Guo, Y., Garner, T. P., Goodwin, O. Y., Henderson, G., Laine, R. A., & Macnaughtan, M. A. (2015). A Kazal-type serine protease inhibitor from the defense gland secretion of the subterranean termite Coptotermes formosanus Shiraki. PLoS One, 10(5), [0125376]. https://doi.org/10.1371/journal.pone.0125376

A Kazal-type serine protease inhibitor from the defense gland secretion of the subterranean termite Coptotermes formosanus Shiraki. / Negulescu, Horia; Guo, Youzhong; Garner, Thomas P.; Goodwin, Octavia Y.; Henderson, Gregg; Laine, Roger A.; Macnaughtan, Megan A.

In: PLoS One, Vol. 10, No. 5, 0125376, 15.05.2015.

Research output: Contribution to journalArticle

Negulescu, H, Guo, Y, Garner, TP, Goodwin, OY, Henderson, G, Laine, RA & Macnaughtan, MA 2015, 'A Kazal-type serine protease inhibitor from the defense gland secretion of the subterranean termite Coptotermes formosanus Shiraki', PLoS One, vol. 10, no. 5, 0125376. https://doi.org/10.1371/journal.pone.0125376
Negulescu, Horia ; Guo, Youzhong ; Garner, Thomas P. ; Goodwin, Octavia Y. ; Henderson, Gregg ; Laine, Roger A. ; Macnaughtan, Megan A. / A Kazal-type serine protease inhibitor from the defense gland secretion of the subterranean termite Coptotermes formosanus Shiraki. In: PLoS One. 2015 ; Vol. 10, No. 5.
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