A human TRIM5α B30.2/SPRY domain mutant gains the ability to restrict and prematurely uncoat B-tropic murine leukemia virus

Felipe Diaz-Griffero, Michel Perron, Kathleen McGee-Estrada, Robert Hanna, Pierre V. Maillard, Didier Trono, Joseph Sodroski

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Human TRIM5α restricts N-tropic murine leukemia virus (N-MLV) but not B-tropic MLV (B-MLV) infection. Here we study B30.2/SPRY domain mutants of human TRIM5α that acquire the ability to inhibit B-MLV infection prior to reverse transcription without losing the ability to restrict N-MLV infection. Remarkably, these mutants gain the ability to decrease the amount of particulate B-MLV capsids in the cytosol of infected cells. In addition, these mutants gain the ability to restrict SIVmac and HIV-2 infection. B-MLV and SIVmac infections were blocked by the mutant TRIM5α proteins prior to reverse transcription. Thus, the range of retroviruses restricted by human TRIM5α can be increased by changes in the B30.2/SPRY domain, which also result in the ability to cause premature uncoating of the restricted retroviral capsid.

Original languageEnglish (US)
Pages (from-to)233-242
Number of pages10
JournalVirology
Volume378
Issue number2
DOIs
StatePublished - Sep 1 2008
Externally publishedYes

Keywords

  • Capsid
  • Mechanism
  • Restriction factor
  • Retrovirus
  • Reverse transcription
  • Tripartite motif
  • Uncoating

ASJC Scopus subject areas

  • Virology

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